Tubulin gamma 1 (TUBG1) antibody

Antigen: Tubulin gamma 1 (TUBG1)

Synonyms: TUBG, GCP-1, TUBGCP1, Tubg, MGC8041, AI451582, AI503389, 1500010O08Rik, Tubg1, MGC55894, MGC174841, zgc:55894, Xgam, Tubg2, TUBG1, MGC142358, LOC542424, TubG1, TUBC, TUBG3, GAMMA-TUBULIN, tug1, Tb03.27F10.510, DKFZp468N0827

Clonality: Monoclonal (TU-30)

Host: Mouse

Reactivity: Human, Pig (Porcine), Mouse (Murine), Rat (Rattus), Cow (Bovine), Chicken, Protozoa, Plant

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN94313

Additional Information

Alternative name: gamma-tubulin

Gene ID: 17q21

Immunogen: C-terminal peptide of gamma-tubulin counjugated to KLH.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: TU-30

Description: The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor memberof tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures,however, serve to provide structural primer for initiation of microtubular nucleation andgrowth, thereby being crutial for microtubule-based cellular processes, above all for mitoticspindle formation. In animal cells, a center of microtubule organization is the centrosomecomposed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar materialcontaining gamma-tubulin. Formation of the mitotic spindle is preceded by duplication ofcentrosome during S phase. Before mitosis, both centrosomes increase their microtubulenucleation capacity and form two microtuble asters that are pushed apart from each other bythe forces of motor proteins associated at the microtubule surface.

Specificity: The antibody TU-30 recognizes C-terminal peptide sequence EYHAATRPDYISWGTQ (aa434-449) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubuleorganizing center (MTOC).

Synonyms: TUBG, GCP-1, TUBGCP1, Tubg, MGC8041, AI451582, AI503389, 1500010O08Rik, Tubg1, MGC55894, MGC174841, zgc:55894, Xgam, Tubg2, TUBG1, MGC142358, LOC542424, TubG1, TUBC, TUBG3, GAMMA-TUBULIN, tug1, Tb03.27F10.510, DKFZp468N0827

Application Details

Application Notes: Immunocytochemistry - Recommended dilution: Purified Antibody: 1-2 µg/ml Staining technique: (a) Fix cells for 10 min in methanol at -20 o C and for 6 min in acetone at -20 o C, (b) Fix cells directly in methanol for 10 min at -20 o C or in acetone for 10 min at -20 o C. Incubation: 45 min in room temperaturePositive control: P-19 mouse embryonal carcinoma cell line3T3 mouse fibroblasts. Application note: The antibody TU-30 stains only fixed cells.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue). Immunofluorescence staining of mouse fibroblasts using anti-gamma-tubulin (TU-30, direct conjugate with Dyomics 547, red). Nuclei were stained with DAPI (blue).

anti-Tubulin gamma 1 (TUBG1) antibody (Image 2)

Publications

Publications: Novuakovua, Druaberovua, Schuermann et al.: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996 (PubMed).

Draberova, Draberova, Surviladze et al.: "Protein tyrosine kinase p53/p56(lyn) forms complexes with gamma-tubulin in rat basophilic leukemia cells." in: International immunology, Vol. 11, Issue 11, pp. 1829-39, 1999 (PubMed).

Binarova P, Cenklova V, Hause B et al.: "Nuclear gamma-tubulin during acentriolar plant mitosis." in: Plant Cell, Vol. 12, Issue 3, pp. 433-42, 2000 (PubMed).

Libusova L, Sulimenko T, Sulimenko V et al.: "gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms." in: Exp Cell Res, Vol. 295, Issue 2, pp. 375-86, 2004 (PubMed).

Mi, Gan, Cheema et al.: "Cancer preventive isothiocyanates induce selective degradation of cellular {alpha}- and {beta}-tubulins by proteasomes." in: The Journal of biological chemistry, Vol. 284, Issue 25, pp. 17039-51, 2009 (PubMed).

Haren, Stearns, Lueders: "Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components." in: PLoS ONE, Vol. 4, Issue 6, pp. e5976, 2009 (PubMed).

Tibelius, Marhold, Zentgraf et al.: "Microcephalin and pericentrin regulate mitotic entry via centrosome-associated Chk1." in: The Journal of cell biology, Vol. 185, Issue 7, pp. 1149-57, 2009 (PubMed).

Mi, Gan, Chung: "Aggresome-like structure induced by isothiocyanates is novel proteasome-dependent degradation machinery." in: Biochemical and biophysical research communications, Vol. 388, Issue 2, pp. 456-62, 2009 (PubMed).

Koledova, Kafkova, Kraemer et al.: "DNA damage-induced degradation of Cdc25A does not lead to inhibition of Cdk2 activity in mouse embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 3, pp. 450-61, 2010 (PubMed).

Löffler, Fechter, Matuszewska et al.: "Cep63 recruits Cdk1 to the centrosome: implications for regulation of mitotic entry, centrosome amplification, and genome maintenance." in: Cancer research, Vol. 71, Issue 6, pp. 2129-39, 2011 (PubMed).

Vulprecht, David, Tibelius et al.: "STIL is required for centriole duplication in human cells." in: Journal of cell science, 2012 (PubMed).