HLA-DR1 (HLA-DR1) antibody

Antigen: HLA-DR1 (HLA-DR1)

Clonality: Monoclonal (MEM-267)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA

Catalog no.: ABIN94350

Additional Information

Alternative name: HLA-DR1 (empty)

Immunogen: Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.

Format: Purified

Isotype: IgG2b  (Matching secondary antibodies)

Clone: MEM-267

Description: HLA-DR1 belongs to the HLA class II beta chain paralogues. The MHC Class II molecule isa heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in themembrane. It plays a central role in the immune system by presenting peptides derived fromextracellular proteins. MHC Class II molecules are expressed in antigen presenting cells(APC). The beta chain is approximately 26-28 kDa. Within the DR molecule the beta chaincontains all the polymorphisms specifying the peptide binding specificities. Hundreds ofDRB1 alleles have been described and typing for these polymorphisms is routinely done forbone marrow and kidney transplantation.

Specificity: The antibody MEM-267 specifically binds to the empty but not peptide-loaded form ofHLA-DR1. DR is the isotypes of human MHC Class II molecules expressed onantigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).

Application Details

Application Notes: Flow Cytometry: The antibody MEM-267 stains immature dendritic cells that express empty cell surface MHCmolecules, but not cells that express predominantly peptide loaded forms.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Western blotting analysis of HLA-DR1 in Raji (A) and Jurkat (B) cell lines using MEM-267 antibody. 

Publications

Publications: Carven GJ, Chitta S, Hilgert I et al.: "Monoclonal antibodies specific for the empty conformation of HLA-DR1 reveal aspects of the conformational change associated with peptide binding." in: J Biol Chem, Vol. 279, Issue 16, pp. 16561-70, 2004 (PubMed).