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HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Heavy Chain) antibody

Details for Product No. ABIN94366, Supplier: Login to see
Antigen
  • MHC-G
  • B-F
  • B-F-S04
  • B-F-S05
  • B-F-S06
  • B-F-S07
  • B-FI
  • B-FIV
  • BF2
  • BFa2
  • BFw-03
  • BFw-05
  • BFz-01
Epitope
Heavy Chain
32
17
8
7
7
7
6
6
6
6
5
2
2
2
2
1
1
1
1
1
Reactivity
Human
368
8
7
3
1
Host
Mouse
237
107
27
6
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
45
41
29
22
13
5
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
177
167
162
82
61
51
45
41
35
32
17
7
2
2
1
1
1
1
Supplier
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Immunogen Denatured bacterially expressed recombinant human HLA-G heavy chain.
Clone MEM-G-1
Isotype IgG1
Specificity The antibody MEM-G/1 reacts with denaturated HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name HLAG (HLAG Antibody Abstract)
Research Area Major Histocompatibility Complex (MHC)
Pathways
Application Notes Immunohistochemistry (paraffin sections) Recommended dilution: 1: 60 to 1: 00
Incubation: 1 h at RT
Positive tissue: Human placenta - extravillous cytotrophoblast
Staining technique: Standard ABC technique
Pretreatment: Heat retrieval in 0.01M citrate buffer (4x2 min. in microwave oven)

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Immunohistochemistry (IHC) image for anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Heavy Chain) antibody (ABIN94366) Immunohistochemistry staining with anti-human HLA-G (MEM-G/1). Fig. 1A - pulmonary di...
Immunohistochemistry (IHC) image for anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (Heavy Chain) antibody (ABIN94366) Immunohistochemistry staining with anti-human HLA-G (MEM-G/1). Fig. 1A - pulmonary di...
Product cited in: Jasinski-Bergner, Stehle, Gonschorek et al.: "Identification of 14-3-3? Gene as a Novel miR-152 Target Using a Proteome-based Approach." in: The Journal of biological chemistry, Vol. 289, Issue 45, pp. 31121-35, 2014 (PubMed).

Amiot, Vu, Rauch et al.: "Expression of HLA-G by mast cells is associated with hepatitis C virus-induced liver fibrosis." in: Journal of hepatology, Vol. 60, Issue 2, pp. 245-52, 2014 (PubMed).

Larsen, Bzorek, Pass et al.: "Human leukocyte antigen-G in the male reproductive system and in seminal plasma." in: Molecular human reproduction, Vol. 17, Issue 12, pp. 727-38, 2011 (PubMed). Further details: incubated over night at room temperature, with primary antibody MEM- G1 in a concentration of 10 ?g/ml, diluted in wash buffer

Biondi, Ferretti, Lunghi et al.: "cAMP efflux from human trophoblast cell lines: a role for Multidrug Resistance Protein (MRP)1 transporter." in: Molecular human reproduction, 2010 (PubMed).

Gonen-Gross, Achdout, Arnon et al.: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, 2005 (PubMed).

Shih, Kurman: "p63 expression is useful in the distinction of epithelioid trophoblastic and placental site trophoblastic tumors by profiling trophoblastic subpopulations." in: The American journal of surgical pathology, Vol. 28, Issue 9, pp. 1177-83, 2004 (PubMed).

Boyson, Erskine, Whitman et al.: "Disulfide bond-mediated dimerization of HLA-G on the cell surface." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 25, pp. 16180-5, 2002 (PubMed).

Hurks, Valter, Wilson et al.: "Uveal melanoma: no expression of HLA-G." in: Investigative ophthalmology & visual science, Vol. 42, Issue 13, pp. 3081-4, 2001 (PubMed).

Frumento, Franchello, Palmisano et al.: "Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment." in: Tissue antigens, Vol. 56, Issue 1, pp. 30-7, 2000 (PubMed).

Fournel, Huc, Aguerre-Girr et al.: "Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules." in: Tissue antigens, Vol. 55, Issue 6, pp. 510-8, 2000 (PubMed).