Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

HLAG antibody (HLA Class I Histocompatibility Antigen, alpha Chain G) (APC) Primary Antibody

HLAG Reactivity: Human ELISA, FACS, ICC, IHC (fro), IP Host: Mouse Monoclonal MEM-G-9 APC
Pubmed (8)
Catalog No. ABIN125708
$651.22
Plus shipping costs $45.00
0.1 mg
local_shipping Shipping to: United States
Delivery in 5 to 7 Business Days
  • Target See all HLAG Antibodies
    HLAG
    Reactivity
    • 136
    • 8
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 67
    • 58
    • 21
    • 1
    • 1
    Mouse
    Clonality
    • 90
    • 58
    Monoclonal
    Conjugate
    • 53
    • 20
    • 16
    • 13
    • 12
    • 7
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HLAG antibody is conjugated to APC
    Application
    • 87
    • 73
    • 68
    • 54
    • 26
    • 23
    • 14
    • 13
    • 13
    • 13
    • 9
    • 7
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
    Specificity
    The antibody MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported.
    The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
    No Cross-Reactivity
    Mouse (Murine)
    Cross-Reactivity (Details)
    Human
    Immunogen
    Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
    Clone
    MEM-G-9
    Isotype
    IgG1
  • Application Notes
    The reagent is designed for Flow Cytometry analysis of cells expressing HLA-G molecule on the cell surface. Suggested working dilution is 1:100. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
    Comment

    The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Avoid prolonged exposure to light.
    Storage
    4 °C
    Storage Comment
    Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
  • Zhao, Teklemariam, Hantash: "Reassessment of HLA-G isoform specificity of MEM-G/9 and 4H84 monoclonal antibodies." in: Tissue antigens, Vol. 80, Issue 3, pp. 231-8, 2012 (PubMed).

    López, Alegre, LeMaoult, Carosella, González: "Regulatory role of tryptophan degradation pathway in HLA-G expression by human monocyte-derived dendritic cells." in: Molecular immunology, Vol. 43, Issue 14, pp. 2151-60, 2006 (PubMed).

    Gonen-Gross, Achdout, Arnon, Gazit, Stern, Horejsí, Goldman-Wohl, Yagel, Mandelboim: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, 2005 (PubMed).

    Menier, Saez, Horejsi, Martinozzi, Krawice-Radanne, Bruel, Le Danff, Reboul, Hilgert, Rabreau, Larrad, Pla, Carosella, Rouas-Freiss: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, 2003 (PubMed).

    Lozano, González, Kindelán, Rouas-Freiss, Caballos, Dausset, Carosella, Peña: "Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule." in: AIDS, Vol. 16, Issue 3, pp. 347-51, 2002 (PubMed).

    Pangault, Le Friec, Caulet-Maugendre, Léna, Amiot, Guilloux, Onno, Fauchet: "Lung macrophages and dendritic cells express HLA-G molecules in pulmonary diseases." in: Human immunology, Vol. 63, Issue 2, pp. 83-90, 2002 (PubMed).

    Fuzzi, Rizzo, Criscuoli, Noci, Melchiorri, Scarselli, Bencini, Menicucci, Baricordi: "HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy." in: European journal of immunology, Vol. 32, Issue 2, pp. 311-5, 2002 (PubMed).

    Fournel, Huc, Aguerre-Girr, Solier, Legros, Praud-Brethenou, Moussa, Chaouat, Berrebi, Bensussan, Lenfant, Le Bouteiller: "Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules." in: Tissue antigens, Vol. 55, Issue 6, pp. 510-8, 2000 (PubMed).

  • Target
    HLAG
    Alternative Name
    HLA-G (HLAG Products)
    Synonyms
    MHC-G, B-F, B-F-S04, B-F-S05, B-F-S06, B-F-S07, B-FI, B-FIV, BF2, BFa2, BFw-03, BFw-05, BFz-01, major histocompatibility complex, class I, G, MHC BF1 class I, HLA-G, BF1
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
You are here:
help Support