HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (FITC)

Details for Product No. ABIN94372
Antigen
Reactivity
Human
323
9
8
5
1
1
1
1
Host
Mouse
219
82
26
5
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
42
41
25
18
11
5
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
184
171
170
110
62
47
36
32
22
18
14
13
8
3
2
2
2
1
1
Options
Immunogen Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
Clone MEM-G-9
Isotype IgG1
Specificity The antibody MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported.
The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
No Cross-Reactivity Mouse (Murine)
Cross-Reactivity (Details) Human
Antigen
Alternative Name HLA-G (HLAG Antibody Abstract)
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
Application Notes The reagent is designed for Flow Cytometry analysis of cells expressing HLA-G molecule on the cell surface. Suggested working dilution is 1:500. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (FITC) (ABIN94372) Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human ...
Image no. 2 for anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (FITC) (ABIN94372) Immunofluorescence staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-human HLA...
Image no. 3 for anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (FITC) (ABIN94372) Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA...
Product cited in: Zhao, Teklemariam, Hantash: "Reassessment of HLA-G isoform specificity of MEM-G/9 and 4H84 monoclonal antibodies." in: Tissue antigens, Vol. 80, Issue 3, pp. 231-8, 2012 (PubMed).

Rizzo, Lanzoni, Stignani, Campioni, Alviano, Ricci, Tazzari, Melchiorri, Scalinci, Cuneo, Bonsi, Lanza, Bagnara, Baricordi: "A simple method for identifying bone marrow mesenchymal stromal cells with a high immunosuppressive potential." in: Cytotherapy, 2010 (PubMed).

López, Alegre, LeMaoult, Carosella, González: "Regulatory role of tryptophan degradation pathway in HLA-G expression by human monocyte-derived dendritic cells." in: Molecular immunology, Vol. 43, Issue 14, pp. 2151-60, 2006 (PubMed).

Gonen-Gross, Achdout, Arnon, Gazit, Stern, Horejsí, Goldman-Wohl, Yagel, Mandelboim: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, 2005 (PubMed).

Menier, Saez, Horejsi, Martinozzi, Krawice-Radanne, Bruel, Le Danff, Reboul, Hilgert, Rabreau, Larrad, Pla, Carosella, Rouas-Freiss: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, 2003 (PubMed).

Lozano, González, Kindelán, Rouas-Freiss, Caballos, Dausset, Carosella, Peña: "Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule." in: AIDS, Vol. 16, Issue 3, pp. 347-51, 2002 (PubMed).

Pangault, Le Friec, Caulet-Maugendre, Léna, Amiot, Guilloux, Onno, Fauchet: "Lung macrophages and dendritic cells express HLA-G molecules in pulmonary diseases." in: Human immunology, Vol. 63, Issue 2, pp. 83-90, 2002 (PubMed).

Fuzzi, Rizzo, Criscuoli, Noci, Melchiorri, Scarselli, Bencini, Menicucci, Baricordi: "HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy." in: European journal of immunology, Vol. 32, Issue 2, pp. 311-5, 2002 (PubMed).

Fournel, Huc, Aguerre-Girr, Solier, Legros, Praud-Brethenou, Moussa, Chaouat, Berrebi, Bensussan, Lenfant, Le Bouteiller: "Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules." in: Tissue antigens, Vol. 55, Issue 6, pp. 510-8, 2000 (PubMed).