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HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

Details for Product No. ABIN94369
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Antigen
Synonyms MHC-G, B-F, B-F-S04, B-F-S05, B-F-S06, B-F-S07, B-FI, B-FIV, BF2, BFa2, BFw-03, BFw-05, BFz-01
Reactivity
Human
(249), (4)
Host
Mouse
(176), (76), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(25), (25), (20), (9), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)
(160), (96), (76), (49), (48), (42), (39), (26), (23), (15), (4), (1), (1), (1)
Pubmed 14 references available
Quantity 0.1 mg
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Catalog No. ABIN94369
348.48 $
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Immunogen Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
Clone MEM-G-9
Isotype IgG1
Specificity The antibody MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
No Cross-Reactivity Mouse (Murine)
Purification Purified by protein A
Purity > 95 % (by SDS-PAGE)
Alternative Name HLAG
Research Area Major Histocompatibility Complex (MHC)
Application Notes Flow Cytometry: Recommended dilution: 1-5 µg/mL
Positive control: JEG-3 human choriocarcinoma cell line
Immunocytochemistry: Recommended dilution: Alexa Fluor®, 488 Fab-fragment: 5 µg/mL
ELISA:
Application note: The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32. Coating antibody (10 µg/mL )Detection antibody (biotin or peroxidase conjugate, 1 µg/mL )

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) and protein G. HLA-G was detected by anti-human HLA-G (4H84, ABIN192389) and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (2) Immunofluorescence staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-human HLA-G (MEM-G/9) Alexa Fluor® 488 Fab-fragment.
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (3) Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC (ABIN94377).
Product cited in: Alegre, Howangyin, Favier et al.: "Membrane redistributions through multi-intercellular exchanges and serial trogocytosis." in: Cell research, Vol. 20, Issue 11, pp. 1239-51, 2010 (PubMed).

Gonzalez, Alegre, Arroyo et al.: "Identification of circulating nonclassic human leukocyte antigen G (HLA-G)-like molecules in exudates." in: Clinical chemistry, Vol. 57, Issue 7, pp. 1013-22, 2011 (PubMed).

Giuliani, Fleury, Vernochet et al.: "Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation." in: PLoS ONE, Vol. 6, Issue 5, pp. e19988, 2011 (PubMed).

General Fournel, Huc, Aguerre-Girr et al.: "Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules." in: Tissue antigens, Vol. 55, Issue 6, pp. 510-8, 2000 (PubMed).

Fuzzi, Rizzo, Criscuoli et al.: "HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy." in: European journal of immunology, Vol. 32, Issue 2, pp. 311-5, 2002 (PubMed).

Pangault, Le Friec, Caulet-Maugendre et al.: "Lung macrophages and dendritic cells express HLA-G molecules in pulmonary diseases." in: Human immunology, Vol. 63, Issue 2, pp. 83-90, 2002 (PubMed).

Lozano, González, Kindelán et al.: "Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule." in: AIDS (London, England), Vol. 16, Issue 3, pp. 347-51, 2002 (PubMed).

Menier, Saez, Horejsi et al.: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, 2003 (PubMed).

Gonen-Gross, Achdout, Arnon et al.: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, 2005 (PubMed).

López, Alegre, LeMaoult et al.: "Regulatory role of tryptophan degradation pathway in HLA-G expression by human monocyte-derived dendritic cells." in: Molecular immunology, Vol. 43, Issue 14, pp. 2151-60, 2006 (PubMed).

Ongaro, Stignani, Pellati et al.: "Human leukocyte antigen-G molecules are constitutively expressed by synovial fibroblasts and upmodulated in osteoarthritis." in: Human immunology, Vol. 71, Issue 4, pp. 342-50, 2010 (PubMed).

Shaikly, Shakhawat, Withey et al.: "Cell bio-imaging reveals co-expression of HLA-G and HLA-E in human preimplantation embryos." in: Reproductive biomedicine online, Vol. 20, Issue 2, pp. 223-233, 2010 (PubMed).

Morandi, Ferretti, Bocca et al.: "A novel mechanism of soluble HLA-G mediated immune modulation: downregulation of T cell chemokine receptor expression and impairment of chemotaxis." in: PLoS ONE, Vol. 5, Issue 7, pp. e11763, 2010 (PubMed).

Gauster, Berghold, Moser et al.: "Fibulin-5 expression in the human placenta." in: Histochemistry and cell biology, Vol. 135, Issue 2, pp. 203-13, 2011 (PubMed).

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