Immunoglobulin G F(ab)2 Fragment (IgG) antibody

Antigen: Immunoglobulin G F(ab)2 Fragment (IgG)

Clonality: Monoclonal (4A11)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA

Catalog no.: ABIN94400

Additional Information

Alternative name: IgG (Fab)

Immunogen: Purified human IgG.

Format: Purified

Isotype: IgG2b

Clone: 4A11

Description: Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effectormolecule of the humoral immune response in man. Its concentration in blood plasma ofhealthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasmaimmunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days)and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rateas other immunoglobulins.

Specificity: The antibody 4A11 reacts with Fab fragment of human IgG.

Application Details

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by precipitation methods and ion exchange chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8°C. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. References: *Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95. *Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71. *Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34. *Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32.

Restrictions: For Research Use only

Images

Western Blotting