IgG Light Chain antibody

Antigen: IgG Light Chain

Clonality: Monoclonal (IVA285-1)

Host: Mouse

Reactivity: Cow (Bovine)

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN94401

Additional Information

Immunogen: Fraction of Bovine immunoglobulin.

Format: Purified

Isotype: IgG1

Clone: IVA285-1

Specificity: The antibody IVA285-1 reacts with bovine IgG (light chains). IgG light chains (kappa nadlambda types) consist of a variable region and a constant region and can easily bedifferentiated by the antigenic properties of the constant region.

Application Details

Application Notes: Western Blotting - Recommended dilution: 0,5-1 µg/ml, overnight in 4 o C Positive control and. Sample preparation: BS (bovine serum), dilution 1: 50 in Laemmlireducing buffer, boiled in water bath for 3 minApplication notes: Strongly reacts with bovine IgG light chains, weakly reacts with IgM. Reducing conditions. SDS-PAGE (12% separating gel). Immunohistochemistry (frozen sections)

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Detection of IgG light chain in reduced samples of Fetal Calf Serum (left lane) and Bovine Serum (right lane) by antibody IVA285-1. This antibody is a suitable tool for in-house quality controls of Fetal Calf Serum (Hybridoma Cell Culture grade).