Neurofilament Heavy Protein antibody

Antigen: Neurofilament Heavy Protein

Clonality: Monoclonal (NF-01)

Host: Mouse

Reactivity: Mammalian

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN94433

Additional Information

Gene ID: 22q12.2

Immunogen: Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: NF-01

Description: Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusivelyin neuronal cells, and in those cells most prominently in large axons. NFs in mostvertebrates are composed of three different polypeptide chains with different molecularweights neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L),which share sequence and structural similarity in a coiled-coil core domain, but differ in thelength and sequence of their N-termini and more dramatically of their C-termini which in thecase of NF-M and NF-H form the flexible extensions that link NFs to each other and to otherelements in the cytoplasm. The protein segment on the C-terminal side of the human NF-Hrod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highlycharged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine,methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequencethat covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP)greater than 40 times. Plasma neurofilament heavy chain level has been proposed as amarker of axonal injury and clinical use of its degeneration and loss has been suggested asa biomarker of several neurodegenerative diseases.

Specificity: The antibody NF-01 recognizes a phosphorylated epitope on heavy neurofilament protein(210 kDa) of various species. Antibodies to the various neurofilament subunits are veryuseful cell type markers since the proteins are among the most abundant of the nervoussystem, are expressed only in neurons and are biochemically very stable.

Application Details

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Immunohistochemistry of paraffin-embedded sections (human cerebellum)