Phosphotyrosine (0) antibody
| Antigen |
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| Clonality |
Monoclonal (P-Tyr-01) |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunocytochemistry (ICC)
Western Blotting (WB) (139), Immunoprecipitation (IP) (88), ELISA (61), Enzyme Immunoassay (EIA) (37), Immunofluorescence (IF) (33), Immunohistochemistry (IHC) (21), Flow Cytometry (FACS) (20), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), Immunocytochemistry (ICC) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), To be determined by user (TBD) (2), Radioimmunoassay (RIA) (1)
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| Catalog no. |
ABIN94452 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
299.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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Phosphotyrosine
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Immunogen
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Phosphotyrosine conjugated to bovine serum albumin.
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Format
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Purified
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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P-Tyr-01
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Specificity
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The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.
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Application Notes
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Western Blotting - Recommended dilution: 1-2 µg/ml
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from ascites by protein-A affinity chromatography.
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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Signaling, Metabolism, Amino Acids, Protein Modifications
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Restrictions
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For Research Use only
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Publications
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Brdicka, Imrich, Angelisovua et al.: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).
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Alternatives for antigen "Phosphotyrosine (0)", type "Antibodies"
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Hosts
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Mouse (137), Rabbit (31), Armenian Hamster (2), Goat (2)
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Reactivities
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Human (22), Rat (Rattus) (18), Dog (Canine) (13), Mouse (Murine) (13), Various Species (3), All Species (2), Chicken (1), Cow (Bovine) (1)
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Applications
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Western Blotting (WB) (139), Immunoprecipitation (IP) (88), ELISA (61), Enzyme Immunoassay (EIA) (37), Immunofluorescence (IF) (33), Immunohistochemistry (IHC) (21), Flow Cytometry (FACS) (20), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), Immunocytochemistry (ICC) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), To be determined by user (TBD) (2), Radioimmunoassay (RIA) (1)
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Conjugates
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Biotin (26), HRP (18), FITC (14), PE (7), Agarose Beads (5), Alkaline Phosphatase (AP) (5), RPE (2), TAMRA (2), Rhodamine (1)
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