SLP65 / BLNK antibody

Antigen: SLP65 / BLNK

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: Western Blotting (WB)

Catalog no.: ABIN94495

Additional Information

Immunogen: A fusion protein representing amino acids 171-356 of human BLNK.

Format: Purified

Description: SLP65 / BLNK (SH2 domain-containing leukocyte-specific phosphoprotein of 65 kDa, B celllinker protein), also known as BASH, is an adaptor protein that plays key role in B cellactivation initiated by cross-linking the B cell receptor (BCR). Phosphorylated by Syktyrosine kinase, SLP65 serves as a scaffold for Btk tyrosine kinase, Vav1 guaninenucleotide exchange factor, phospholipase C gamma2, as well as Grb2 and Nck adaptorproteins, thus represents a central linker protein that bridges the BCR-associated kinaseswith a multitude of signaling pathways.

Specificity: The polyclonal antibody reacts with mouse SLP65 / BLNK, a cytosolic adaptor proteinidentified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternativelyspliced forms of human SLP65).

Application Details

Application Notes: Western Blotting. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withreducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from rabbit serum by precipitation methods.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Western Blotting