Alanine Transaminase antibody

Antigen: Alanine Transaminase

Clonality: Polyclonal

Host: Sheep

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine), Pig (Porcine)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN94798

Additional Information

Alternative name: Alanine Transaminase (ALT)

Immunogen: Alanine Transaminase (ALT) [Pig Heart] * Alanine Transaminase (ALT) is also referred to as Glutamic Pyruvic Transaminase (GPT)

Format: Lyophilized

Specificity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alanine Transaminase (ALT) [Pig Heart]. Cross reactivity against Alanine Transaminase (ALT) from other tissues and species may occur but have not been specifically determined.

Application Details

Application Notes: Recommended Dilutions: This product has been assayed against 1.0 ug of Alanine Transaminase (ALT) [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code ABIN101990 and (ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.

Concentration: 96.0 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of restoration.

Research Area: Enzymes

Restrictions: For Research Use only