Alcohol Dehydrogenase (ADH) antibody

Antigen: Alcohol Dehydrogenase (ADH)

Synonyms: ALCOHOL DEHYDROGENASE, ARABIDOPSIS TAPETUM 1, ASD, ATA1, ADH, ADH1, ALCOHOL DEHYDROGENASE 1, ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE, ATADH, ATADH1, F22K20.19, F22K20_19, BG:DS01486.8, CG32954, DmADH, Dreg-1, Reg-1, T16, dADH, DmelCG3481, CG3481, Adh-2, dvir_GLEANR_2772, DvirGJ18209, GJ18209, Adh2, Adh-1, DvirAdh, dvir_GLEANR_2771, DvirGJ18208, GJ18208, Adh1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Saccharomyces cerevisiae (S. cerevisiae)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN94889

Additional Information

Swiss-Prot: P00330

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with full length Alcohol Dehydrogenase isolated from yeast.

Format: Lyophilized

Description: Gene: ADH1, ADC1, YOL086C. Synonyms: Alcohol dehydrogenase 1 EC=1.1.1.1. Alcohol dehydrogenase I YADH-1. Background: Alcohol dehydrogenase is an isozyme that preferentially catalyzes the conversion of acetaldehyde to acetone. Alcohol dehydrogenase has an apparent molecular weight of 37 kDa (monomer subunit) and forms a homotetramer. This enzyme acts on a variety of primary unbranched aliphatic alcohols and requires 2 bound zinc ions per subunit. Alcohol dehydrogenase shows a cytoplasmic localization. Microheterogeneities may also occur at positions 137, 138, 242-244, and 255 and near position 287.

Specificity: Cross reactivity against Alcohol Dehydrogenase from most fungal sources is likely due to sequence homology as determined by BLAST analysis. Cross reactivity with Alcohol Dehydrogenase from other sources is unknown.

Synonyms: ALCOHOL DEHYDROGENASE, ARABIDOPSIS TAPETUM 1, ASD, ATA1, ADH, ADH1, ALCOHOL DEHYDROGENASE 1, ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE, ATADH, ATADH1, F22K20.19, F22K20_19, BG:DS01486.8, CG32954, DmADH, Dreg-1, Reg-1, T16, dADH, DmelCG3481, CG3481, Adh-2, dvir_GLEANR_2772, DvirGJ18209, GJ18209, Adh2, Adh-1, DvirAdh, dvir_GLEANR_2771, DvirGJ18208, GJ18208, Adh1

Application Details

Application Notes: This antibody has been tested for use in ELISA and by western blot. ELISA: 1:5,000 - 1:25,000. WB: 1:500 - 1:2,000. IP: 1:100.

Concentration: 90.0 mg/ml (by Refractometry)

Purity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alcohol Dehydrogenase [Yeast].

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 0.01% (w/v) Sodium Azide.

Storage: Store vial at 4° C prior to restoration. Restore with 2.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Research Area: Enzymes

Restrictions: For Research Use only

Images

Western blot analysis with Anti-Alcohol Dehydrogenase antibody was used to detect yeast Alcohol Dehydrogenase. Comparison to molecular weight markers (not shown) indicates estimated molecular weights consistent with monomer, dimer and tetramer present in this preparation. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] (ABIN102013) diluted 1:5,000 for 45 min at room temperature. The IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.