CDT2/RAMP antibody (Middle Region)

Catalog No. ABIN951451

This Rabbit Polyclonal antibody specifically detects CDT2/RAMP in WB, FACS and EIA. It exhibits reactivity toward Human and Mouseand has been mentioned in 1 publication.

Quick Overview for CDT2/RAMP antibody (Middle Region) (ABIN951451)

Target: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDT2/RAMP antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Product Details anti-CDT2/RAMP Antibody

Binding Specificity: AA 229-256, Middle Region

Specificity: This antibody recognizes Human and Mouse CDT2 / DTL (Center).

Purification: Affinity Chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide between 229-256 amino acids from the Central region of Human DTL.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CDT2/RAMP antibody (ABIN951451)

Abbas, Sivaprasad, Terai, Amador, Pagano, Dutta: "PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex." in: Genes & development, Vol. 22, Issue 18, pp. 2496-506, (2008) (PubMed).

Target Details for CDT2/RAMP

Target: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Alternative Name: CDT2 / DTL

Background: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.Synonyms: CDW1, DCAF2, Ddb1- and Cul4-associated factor 2, Denticleless protein homolog, L2DTL, Lethal(2) denticleless protein homolog, RA regulated Nuclear Matrix Associated Protein, RAMP, Retinoic acid-regulated nuclear matrix-associated protein

Molecular Weight: 79468 Da

Gene ID: 51514

NCBI Accession: NP_057532