CYP27B1 antibody (C-Term)

Catalog No. ABIN951779

The Rabbit Polyclonal anti-CYP27B1 antibody has been validated for WB, IHC (p) and FACS. It is suitable to detect CYP27B1 in samples from Human.

Quick Overview for CYP27B1 antibody (C-Term) (ABIN951779)

Target: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP27B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-CYP27B1 Antibody

Binding Specificity: AA 479-508, C-Term

Specificity: Recognizes CYP27B1 (C-term).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide between 479~508 amino acids from the C-terminal region of Human CYP27B1

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, 0.09 % (W/V) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CYP27B1

Target: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Alternative Name: CYP27B1

Background: CYP27B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.Synonyms: 25-OHD-1 alpha-hydroxylase, 25-hydroxyvitamin D(3) 1-alpha-hydroxylase, 25-hydroxyvitamin D-1 alpha hydroxylase, CYP1ALPHA, CYP27B, Calcidiol 1-monooxygenase, Cytochrome P450 subfamily XXVIIB polypeptide 1, Cytochrome P450C1 alpha, Cytochrome P450VD1-alpha, Cytochrome p450 27B1, VD3 1A hydroxylase

Gene ID: 1594

NCBI Accession: NP_000776

Pathways: Metabolism of Steroid Hormones and Vitamin D