SREBF2 antibody (Middle Region)

Catalog No. ABIN954950

Product Details anti-SREBF2 Antibody

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Binding Specificity: AA 402-431, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SREBF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody recognizes SREBF2 / SREBP2.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Purified through a Protein A column followed by peptide affinity purification

Immunogen: Synthetic peptide - KLH conjugated - corresponding to the central region (between 402-431aa) of human SREBF2 / SREBP2

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SREBF2

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Alternative Name: SREBF2 / SREBP2 (SREBF2 Products)

Background: SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism. Each SREBP consists of three domains, including an amino-terminal transcription factor domain of ~480 amino acids, a middle hydrophobic region of ~80 amino acids containing two hydrophobic transmembrane segments, and a carboxy-terminal regulatory domain of ~590 amino acids. SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway. SREBP-2 is ubiquitously detected in various tissues. Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol. LS-B1609 polyclonal antibody detects both precursor and active forms of the protein in tissues and cells such as liver, brown fat, testis, Hep-G2 cells, and human fibroblast. The apparent molecular weight on SDS-PAGE may be higher than the calculated molecular weight (about 126 kDa) due to glycosylation of the protein.Synonyms: BHLHD2, Class D basic helix-loop-helix protein 2, SREBP-2, Sterol regulatory element-binding protein 2, Sterol regulatory element-binding transcription factor 2

Gene ID: 6721

NCBI Accession: NP_004590

Pathways: Regulation of Lipid Metabolism by PPARalpha