Interferon gamma (IFNG) antibody

Details for Product No. ABIN964736
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Antigen
Synonyms IFNG2, IFN-g, Ifg, IFN-G, IFN-gamma, IFNG, IFG, IFI
Reactivity
Cow (Bovine)
(356), (158), (87), (26), (20), (15), (10), (9), (7), (5), (5), (5), (4), (4), (3), (3), (1), (1)
Host
Rabbit
(297), (204), (101), (19), (5), (4), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(78), (53), (22), (14), (13), (10), (5), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application
Western Blotting (WB), ELISA
(391), (333), (171), (74), (60), (58), (57), (48), (44), (37), (30), (29), (19), (11), (10), (9), (7), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 25 µL
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Catalog No. ABIN964736
126.50 $
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Immunogen This protein A purified antibody was prepared prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to the 143 amino acids of the mature Bovine IFN Gamma protein.
Immunogen Type: RecombinantProtein
Isotype IgG
Specificity This product was protein A purified from monospecific antiserum by chromotography. This antibody is specific for bovine IFN gamma protein. A BLAST analysis was used to suggest cross-reactivity with IFN gamma from bovine and red deer sources based on 100% homology with the immunizing sequence. Cross-reactivity with IFN gamma from other sources has not been determined.
Characteristics Interferon-gamma (IFN-gamma) is a dimerized soluble cytokine that is the only member of the type II class of interferons. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-gamma belongs. IFN-gamma, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN-gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN-gamma in the immune system stems in part from its ability to inhibit viral replication directly, but, most important, derives from its immunostimulatory and immunomodulatory effects. IFN-gamma is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.
Purification purified
Sterility Sterile filtered
Alternative Name Interferon Gamma
Background Interferon-gamma (IFN-gamma) is a dimerized soluble cytokine that is the only member of the type II class of interferons. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-gamma belongs. IFN-gamma, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN-gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN-gamma in the immune system stems in part from its ability to inhibit viral replication directly, but, most important, derives from its immunostimulatory and immunomodulatory effects. IFN-gamma is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.
Synonyms: BoIFNG, IFN, Interferon gamma, IFNG
Gene ID 281237
NCBI Accession NP_776511
UniProt A9QXB7
Application Notes This protein A purified antibody is suitable for ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.9 kDa in size corresponding to bovine IFN gamma by western blotting in the appropriate cell lysate or extract.
Comment

Gene Name: IFN gamma

Restrictions For Research Use only
Format Liquid
Concentration 1.59 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Expiration date is three (3) months from date of opening.
Expiry Date 3 months
Supplier Images
anti-Interferon gamma (IFNG) antibody Western blot using protein-A purified anti-bovine IFN gamma antibody shows detection of recombinant bovine IFN gamma at 16.9 kDa, raised in yeast. Primary antibody was diluted to 1µg/mL. 3% BSA from BSA-30 (Bovine Serum Albumin Solution) was used for blocking. Secondary antibody 611-131-122 (Goat anti-Rabbit IgG IRDye 800) was used at 1:20,000.
Product cited in: Schroder, Hertzog, Ravasi et al.: "Interferon-gamma: an overview of signals, mechanisms and functions." in: Journal of leukocyte biology, Vol. 75, Issue 2, pp. 163-89, 2004 (PubMed).

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