Biotin antibody (DyLight 350)

Catalog No. ABIN965465

Product Details anti-Biotin Antibody (DyLight 350)

Target: Biotin

Reactivity: Please inquire

Host: Goat

Conjugate: This Biotin antibody is conjugated to DyLight 350

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunomicroscopy (IM)

Specificity: This antibody reacts with Biotin

Cross-Reactivity (Details): This antibody does not react with free BSA or free KLH

Characteristics: Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)
Conjugate: DyLight ® 350 (Ex = 353 nm, Em = 432 nm)

Purification: Affinity purified using solid phase biotin

Purity: > 95 % based on SDS-PAGE

Immunogen: BSA-Biotin, KLH-Biotin

Application Details

Application Notes: This Conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications

Comment:

Trademark: DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Concentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store freeze-dried powder at 2-8 °C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.

Target Details for Biotin

Target: Biotin

Abstract: Biotin Products

Target Type: Chemical