BCL antibody

Antigen: BCL

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN965649

Additional Information

Immunogen: This antiserum was produced against synthesized non-phosphopeptide derived from human BCL-2 around the phosphorylation site of serine 70 (R-T-SP-P-L).

Specificity: BCL-2(Ab-70) antibody detects endogenous levels of total BCL-2 protein.

Application Details

Application Notes: IHC: 1:50-1:100.

Concentration: 100ug/100ul.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: 0.02% Sodium Azide

Storage: Store at -20°C/1 year.

Restrictions: For Research Use only

Publications

Product: Yamamoto, Ichijo, Korsmeyer: "BCL-2 is phosphorylated and inactivated by an ASK1/Jun N-terminal protein kinase pathway normally activated at G(2)/M." in: Molecular and cellular biology, Vol. 19, Issue 12, pp. 8469-78, 2000 (PubMed).

Huang, Cidlowski: "Phosphorylation status modulates Bcl-2 function during glucocorticoid-induced apoptosis in T lymphocytes." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 16, Issue 8, pp. 825-32, 2002 (PubMed).

Jin, Gao, Flagg et al.: "Tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone promotes functional cooperation of Bcl2 and c-Myc through phosphorylation in regulating cell survival and proliferation." in: The Journal of biological chemistry, Vol. 279, Issue 38, pp. 40209-19, 2004 (PubMed).