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CD31 antibody (Platelet/endothelial Cell Adhesion Molecule)

Details for Product anti-PECAM1 Antibody No. ABIN965786, Supplier: Log in to see
Antigen
  • CD31
  • CD31/EndoCAM
  • GPIIA'
  • PECA1
  • PECAM-1
  • endoCAM
  • C85791
  • Cd31
  • Pecam
Reactivity
Human
594
265
106
51
19
17
16
15
14
10
5
4
3
1
Host
Mouse
536
189
154
8
7
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD31 antibody is un-conjugated
99
75
74
38
12
10
9
7
6
5
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
520
307
232
152
150
133
113
75
23
21
12
11
11
11
10
10
8
5
5
3
3
3
2
2
2
1
1
1
1
1
1
Supplier
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Immunogen Human leucocytes
Clone B493 (158-2B3)
Isotype IgG1
Purification Concentrated.
Alternative Name CD31 (PECAM-1) (PECAM1 Antibody Abstract)
Gene ID 5175
Pathways
Application Notes Immunohistochemistry: 1:50-1:100.
Flow cytometry: 1:150-1:300
Immunoprecipitation.
Staining Procedure: This antibody can be used on frozen tissue sections. When using with formalin-fixed paraffin-embedded tissue sections an antigen demasking treatment is recommended. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.
Comment

Recommended Positive Control: Tonsil, Thymus, Spleen

Restrictions For Research Use only
Format Liquid
Concentration 0.3 mg/ml.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: van Mourik, Leeksma, Reinders et al.: "Vascular endothelial cells synthesize a plasma membrane protein indistinguishable from the platelet membrane glycoprotein IIa." in: The Journal of biological chemistry, Vol. 260, Issue 20, pp. 11300-6, 1985 (PubMed).

Background publications Albelda, Muller, Buck et al.: "Molecular and cellular properties of PECAM-1 (endoCAM/CD31): a novel vascular cell-cell adhesion molecule." in: The Journal of cell biology, Vol. 114, Issue 5, pp. 1059-68, 1991 (PubMed).

Stockinger, Gadd, Eher et al.: "Molecular characterization and functional analysis of the leukocyte surface protein CD31." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3889-97, 1991 (PubMed).

Newman, Berndt, Gorski et al.: "PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily." in: Science (New York, N.Y.), Vol. 247, Issue 4947, pp. 1219-22, 1990 (PubMed).