anti-CD31 antibody (Platelet/endothelial Cell Adhesion Molecule)

Details for Product anti-PECAM1 Antibody No. ABIN965786
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Antigen
Synonyms CD31, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, endoCAM, C85791, Cd31, Pecam
Reactivity
Human
(586), (301), (100), (53), (21), (16), (16), (10), (10), (5), (3), (2), (2)
Host
Mouse
(523), (200), (180), (8), (8)
Clonality (Clone)
Monoclonal ()
Conjugate
This CD31 antibody is un-conjugated
(106), (81), (79), (40), (13), (11), (10), (6), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)
(563), (311), (219), (160), (134), (133), (116), (81), (37), (26), (11), (11), (10), (10), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 0.5 mL
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Immunogen Human leucocytes
Clone B493 (158-2B3)
Isotype IgG1
Purification Concentrated.
Alternative Name CD31 (PECAM-1) (PECAM1 Antibody Abstract)
Gene ID 5175
Application Notes Immunohistochemistry: 1:50-1:100.
Flow cytometry: 1:150-1:300
Immunoprecipitation.
Staining Procedure: This antibody can be used on frozen tissue sections. When using with formalin-fixed paraffin-embedded tissue sections an antigen demasking treatment is recommended. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.
Comment

Recommended Positive Control: Tonsil, Thymus, Spleen

Restrictions For Research Use only
Format Liquid
Concentration 0.3 mg/ml.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: van Mourik, Leeksma, Reinders et al.: "Vascular endothelial cells synthesize a plasma membrane protein indistinguishable from the platelet membrane glycoprotein IIa." in: The Journal of biological chemistry, Vol. 260, Issue 20, pp. 11300-6, 1985 (PubMed).

Background publications Albelda, Muller, Buck et al.: "Molecular and cellular properties of PECAM-1 (endoCAM/CD31): a novel vascular cell-cell adhesion molecule." in: The Journal of cell biology, Vol. 114, Issue 5, pp. 1059-68, 1991 (PubMed).

Stockinger, Gadd, Eher et al.: "Molecular characterization and functional analysis of the leukocyte surface protein CD31." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3889-97, 1991 (PubMed).

Newman, Berndt, Gorski et al.: "PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily." in: Science (New York, N.Y.), Vol. 247, Issue 4947, pp. 1219-22, 1990 (PubMed).

Validation Images
Catalog No. ABIN965786
206.25 $
Plus shipping costs $45.00
Quantity
Price
0.5 mL
206.25 $

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