elF4E antibody

Antigen: elF4E

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966057

Additional Information

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T).

Specificity: ElF4E (Ab-209) antibody detects endogenous levels of total elF4E protein.

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Concentration: 100ug/100ul.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: 0.02% Sodium Azide

Storage: Store at -20°C/1 year.

Restrictions: For Research Use only

Publications

Product: Ohlmann, Rau, Pain et al.: "The C-terminal domain of eukaryotic protein synthesis initiation factor (eIF) 4G is sufficient to support cap-independent translation in the absence of eIF4E." in: The EMBO journal, Vol. 15, Issue 6, pp. 1371-82, 1996 (PubMed).

Li, McDonald, Nassar et al.: "Clinical outcome in stage I to III breast carcinoma and eIF4E overexpression." in: Annals of surgery, Vol. 227, Issue 5, pp. 756-6l; discussion 761-3, 1998 (PubMed).

Gu, Li, Zhao et al.: "tRNASer(CGA) differentially regulates expression of wild-type and codon-modified papillomavirus L1 genes." in: Nucleic acids research, Vol. 32, Issue 15, pp. 4448-61, 2004 (PubMed).