Chromosome 14 Open Reading Frame 1 (C14orf1) (N-Term) antibody

Antigen: Chromosome 14 Open Reading Frame 1 (C14orf1)

Synonyms: ERG28, NET51, homolog of yeast ergosterol28, T27I1.5, T27I1_5, C7H14orf1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966089

Additional Information

Alternative name: ERG28

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to N-terminal residues of mouse ERG28(Probable ergosterol biosynthetic protein 28)

Description: ERG28(Probable ergosterol biosynthetic protein 28) has a role as a scaffold to help anchor ERG25, ERG26 and ERG27 to the endoplasmic reticulum. ERG28 may also be responsible for facilitating their interaction. The ERG28-encoded protein, Erg28p, interacts with both the sterol C-4 demethylation enzyme complex as well as the late biosynthetic protein, the C-24 sterol methyltransferase (Erg6p). ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation-related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum. ERG28 interacts with ERG25, ERG26 and ERG27. It also interacts with ERG1, ERG3, ERG5, ERG6 and ERG11.

Application Details

Application Notes: ELISA, Western blotting: 1µg/ml for 2hrs.

Purification: Purified by antigen-specific affinity chromatography.

Buffer: This antibody is stored in PBS, 50% glycerol

Preservative: 0.01% Sodium Azide

Storage: Store at -20°C.

Research Area: Signaling, Metabolism

Restrictions: For Research Use only

Publications

Product: Mo, Valachovic, Randall et al.: "Protein-protein interactions among C-4 demethylation enzymes involved in yeast sterol biosynthesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 15, pp. 9739-44, 2002 (PubMed).

Ghaemmaghami, Huh, Bower et al.: "Global analysis of protein expression in yeast." in: Nature, Vol. 425, Issue 6959, pp. 737-41, 2003 (PubMed).

Mo, Valachovic, Bard: "The ERG28-encoded protein, Erg28p, interacts with both the sterol C-4 demethylation enzyme complex as well as the late biosynthetic protein, the C-24 sterol methyltransferase (Erg6p)." in: Biochimica et biophysica acta, Vol. 1686, Issue 1-2, pp. 30-6, 2004 (PubMed).