gadE (N-Term) antibody

Antigen: gadE

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Escherichia coli (E. coli)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966176

Additional Information

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to N-terminal residues of Escherichia coli O157:H7 gadE(Transcriptional regulator gadE)

Description: The gadE(Transcriptional regulator gadE) regulates the expression of several genes involved in acid resistance. GadE is required for the expression of gadA and gadBC, among others, regardless of media or growth conditions. gadE binds directly to the 20 bp GAD box found in the control regions of both loci. The gadE could be involved in the regulation of the genes coding for the type III secretion system in enterohaemorragic strains. The gadE could be induced by evgA via the induction of ydeO. The gadE contains 1 HTH luxR-type DNA-binding domain.

Application Details

Application Notes: ELISA, Western blotting: 1µg/ml for 2hrs.

Purification: Purified by antigen-specific affinity chromatography.

Buffer: This antibody is stored in PBS, 50% glycerol

Preservative: 0.01% Sodium Azide

Storage: Store at -20°C.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors, Bacteria

Restrictions: For Research Use only

Publications

Product: Perna, Plunkett, Burland et al.: "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." in: Nature, Vol. 409, Issue 6819, pp. 529-33, 2001 (PubMed).

Hayashi, Makino, Ohnishi et al.: "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 8, Issue 1, pp. 11-22, 2001 (PubMed).