Green Fluorescent Protein (GFP) (N-Term) antibody

Details for Product No. ABIN966201
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Antigen
Epitope
N-Term
(25), (18), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Aequorea victoria
(341), (31), (11), (4), (3), (3), (2), (1), (1)
Host
Rabbit
(149), (147), (56), (26), (4), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(24), (19), (14), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC)
(301), (191), (107), (61), (59), (47), (38), (29), (29), (16), (13), (10), (8), (4), (3), (2)
Pubmed 7 references available
Catalog no. ABIN966201
Quantity 0.1 mg
Price
397.38 $   Plus shipping costs $45.00
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  • +1 404 474 4654
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Immunogen Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to N-terminal residues of Aequorea victoria GFP (Green fluorescent protein)
Purification Purified by antigen-specific affinity chromatography.
Alternative Name GFP
Background GFP(Green fluorescent protein) is an energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. It contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
Research Area Tags/Labels
Application Notes ELISA, Western blotting: 1µg/ml for 2hrs.
Restrictions For Research Use only
Format Liquid
Buffer This antibody is stored in PBS, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Prasher, Eckenrode, Ward et al.: "Primary structure of the Aequorea victoria green-fluorescent protein." in: Gene, Vol. 111, Issue 2, pp. 229-33, 1992 (PubMed).

Inouye, Noguchi, Sakaki et al.: "Cloning and sequence analysis of cDNA for the luminescent protein aequorin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 10, pp. 3154-8, 1985 (PubMed).

Inouye, Tsuji: "Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein." in: FEBS letters, Vol. 341, Issue 2-3, pp. 277-80, 1994 (PubMed).

Cody, Prasher, Westler et al.: "Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein." in: Biochemistry, Vol. 32, Issue 5, pp. 1212-8, 1993 (PubMed).

Ormö, Cubitt, Kallio et al.: "Crystal structure of the Aequorea victoria green fluorescent protein." in: Science (New York, N.Y.), Vol. 273, Issue 5280, pp. 1392-5, 1996 (PubMed).

Yang, Moss, Phillips: "The molecular structure of green fluorescent protein." in: Nature biotechnology, Vol. 14, Issue 10, pp. 1246-51, 1998 (PubMed).

Wachter, Elsliger, Kallio et al.: "Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein." in: Structure (London, England : 1993), Vol. 6, Issue 10, pp. 1267-77, 1998 (PubMed).

Hosts (149), (147), (56), (26), (4), (2), (1)
Reactivities (341), (31), (11), (4), (3), (3), (2), (1), (1)
Applications (301), (191), (107), (61), (59), (47), (38), (29), (29), (16), (13), (10), (8), (4), (3), (2)
Conjugates (24), (19), (14), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (25), (18), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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