p21 antibody
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- Target See all p21 (CDKN1A) Antibodies
- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This p21 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
- Specificity
- This antibody reacts with human and other mammalian p21 protein. p21 is an intracellular protein of 21kD size. It is also known as the wild type p53 activated fragment 1 or WAF1. p21 inhibits cyclin-dependent kinases (Cdk's) and also proliferating cell nuclear antigens or PCNA's).
- Purification
- Concentrated.
- Immunogen
- Human p21 protein.
- Clone
- B459
- Isotype
- IgG1
- Top Product
- Discover our top product CDKN1A Primary Antibody
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- Application Notes
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Immunohistochemistry: 1:50-1:100 on tissue sections.
Western blotting: 1:100-1:300.
ELISA: 1:500-1:3000.
Staining Procedure: This antibody can be used on frozen and formalin-fixed paraffin-embedded tissue sections. When using on formalin-fixed paraffinembedded tissues a antigen demasking procedure is recommended.The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory. - Comment
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Cellular Localization: Nuclear.
Recommended Positive Control: Breast, Skin, or Colon Carcinoma, Fibroadenoma. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.3 mg/ml.
- Buffer
- 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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Identification of the active region of the DNA synthesis inhibitory gene p21Sdi1/CIP1/WAF1." in: The EMBO journal, Vol. 14, Issue 3, pp. 555-63, (1995) (PubMed).
: "The C-terminal region of p21SDI1/WAF1/CIP1 is involved in proliferating cell nuclear antigen binding but does not appear to be required for growth inhibition." in: The Journal of biological chemistry, Vol. 270, Issue 29, pp. 17060-3, (1995) (PubMed).
: "
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Identification of the active region of the DNA synthesis inhibitory gene p21Sdi1/CIP1/WAF1." in: The EMBO journal, Vol. 14, Issue 3, pp. 555-63, (1995) (PubMed).
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- Target
- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
- Alternative Name
- WAF 1/p21 Protein (CDKN1A Products)
- Molecular Weight
- 21 kDa
- Pathways
- p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy
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