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Details for Product No. ABIN966329

Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) antibody

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Antigen
Synonyms P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1, p21, cip1, p16Xic2, CCND1, sdi1, waf1, cap20, cdkn1, mda-6, p21cip1, CDKN1A, si:ch1073-48m5.2
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (ffpe)), Immunohistochemistry (Frozen Sections) (IHC (fro))
Pubmed 3 references available
Catalog no. ABIN966329
Quantity 0.5 mL
Price
192.50 $   Plus shipping costs $45.00
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Product details

Target Details Application Details Handling References Alternatives back to top
Immunogen Human p21 protein.
Clone B459
Isotype IgG1
Specificity This antibody reacts with human and other mammalian p21 protein. p21 is an intracellular protein of 21kD size. It is also known as the wild type p53 activated fragment 1 or WAF1. p21 inhibits cyclin-dependent kinases (Cdk's) and also proliferating cell nuclear antigens or PCNA's).
Purification Concentrated.

Target Details

Application Details Handling References Alternatives back to top
Alternative Name WAF 1/p21 Protein
Molecular Weight 21 kDa

Application Details

Target Details Handling References Alternatives back to top
Application Notes Immunohistochemistry: 1:50-1:100 on tissue sections.
Western blotting: 1:100-1:300.
ELISA: 1:500-1:3000.
Staining Procedure: This antibody can be used on frozen and formalin-fixed paraffin-embedded tissue sections. When using on formalin-fixed paraffinembedded tissues a antigen demasking procedure is recommended.The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.
Comment

Cellular Localization: Nuclear.
Recommended Positive Control: Breast, Skin, or Colon Carcinoma, Fibroadenoma.
Restrictions For Research Use only

Handling

Target Details Application Details References Alternatives back to top
Concentration 0.3 mg/ml.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Preservative Sodium azide
Storage 4 °C

References

Target Details Application Details Handling Alternatives back to top
Product cited in: Nakanishi, Robetorye, Pereira-Smith et al.: "The C-terminal region of p21SDI1/WAF1/CIP1 is involved in proliferating cell nuclear antigen binding but does not appear to be required for growth inhibition." in: The Journal of biological chemistry, Vol. 270, Issue 29, pp. 17060-3, 1995 (PubMed).

Nakanishi, Robetorye, Adami et al.: "Identification of the active region of the DNA synthesis inhibitory gene p21Sdi1/CIP1/WAF1." in: The EMBO journal, Vol. 14, Issue 3, pp. 555-63, 1995 (PubMed).

el-Deiry, Tokino, Velculescu et al.: "WAF1, a potential mediator of p53 tumor suppression." in: Cell, Vol. 75, Issue 4, pp. 817-25, 1993 (PubMed).

Alternatives

Target Details Application Details Handling References back to top
Alternatives for antigen "Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A)", type "Antibodies"
Hosts (144), (76), (3), (1)
Reactivities (212), (61), (58), (25), (24), (12), (12), (12), (3), (2), (1), (1), (1)
Applications (125), (84), (61), (50), (45), (33), (22), (17), (7), (7), (7), (7), (5), (2), (1)
Conjugates (9), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Epitopes (32), (19), (16), (15), (12), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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