Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) antibody

Details for Product No. ABIN966329
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Antigen
Synonyms p21, cip1, p16Xic2, CCND1, sdi1, waf1, cap20, cdkn1, mda-6, p21cip1, CDKN1A, si:ch1073-48m5.2, Cip1, UV96, Waf1, CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1, CDKI, Cdkn1, mda6, p21Cip1, p21WAF
Reactivity
Human
(289), (87), (59), (24), (24), (12), (12), (12), (1)
Host
Mouse
(238), (76), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(16), (16), (15), (9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections), Immunohistochemistry (Frozen Sections) (IHC (fro))
(188), (101), (82), (77), (50), (49), (39), (31), (15), (13), (10), (6), (5), (5), (2), (2)
Pubmed 3 references available
Quantity 0.5 mL
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Catalog No. ABIN966329
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Immunogen Human p21 protein.
Clone B459
Isotype IgG1
Specificity This antibody reacts with human and other mammalian p21 protein. p21 is an intracellular protein of 21kD size. It is also known as the wild type p53 activated fragment 1 or WAF1. p21 inhibits cyclin-dependent kinases (Cdk's) and also proliferating cell nuclear antigens or PCNA's).
Purification Concentrated.
Alternative Name WAF 1/p21 Protein
Molecular Weight 21 kDa
Application Notes Immunohistochemistry: 1:50-1:100 on tissue sections.
Western blotting: 1:100-1:300.
ELISA: 1:500-1:3000.
Staining Procedure: This antibody can be used on frozen and formalin-fixed paraffin-embedded tissue sections. When using on formalin-fixed paraffinembedded tissues a antigen demasking procedure is recommended.The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.
Comment

Cellular Localization: Nuclear.
Recommended Positive Control: Breast, Skin, or Colon Carcinoma, Fibroadenoma.
Restrictions For Research Use only
Format Liquid
Concentration 0.3 mg/ml.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: el-Deiry, Tokino, Velculescu et al.: "WAF1, a potential mediator of p53 tumor suppression." in: Cell, Vol. 75, Issue 4, pp. 817-25, 1993 (PubMed).

Nakanishi, Robetorye, Adami et al.: "Identification of the active region of the DNA synthesis inhibitory gene p21Sdi1/CIP1/WAF1." in: The EMBO journal, Vol. 14, Issue 3, pp. 555-63, 1995 (PubMed).

Nakanishi, Robetorye, Pereira-Smith et al.: "The C-terminal region of p21SDI1/WAF1/CIP1 is involved in proliferating cell nuclear antigen binding but does not appear to be required for growth inhibition." in: The Journal of biological chemistry, Vol. 270, Issue 29, pp. 17060-3, 1995 (PubMed).

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