CDKN1A antibody (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1))

Details for Product anti-CDKN1A Antibody No. ABIN94444
Antigen
Reactivity
Human, Non-Human Primate
476
180
120
47
39
7
6
5
4
1
1
1
1
1
1
Host
Mouse
339
179
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CDKN1A antibody is un-conjugated
31
22
22
15
15
15
9
9
9
9
9
9
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
269
170
165
152
122
80
68
65
53
28
18
16
9
6
5
2
2
1
Options
Immunogen Human p21 protein
Clone WA-1
Isotype IgG1
Specificity The antibody WA-1 reacts with p21 protein (Waf1, Cip1, SDI 1), a 21 kDa tumour suppressor, which inhibits the activity of cyclin-dependent family kinases and of proliferating cell nuclear antigen.
Cross-Reactivity (Details) Human, Non-Human Primates
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name p21Waf1 (CDKN1A Antibody Abstract)
Background The tumour suppressor p21Waf1 (Wild-type p53-Activated Fragment 1, also known as Cip1, Cdk interacting protein, or SDI 1) is a cyclin-dependent kinase (Cdk) inhibitor, which is expressed by involvement of p53, Egr-1, AP2, STATs or other transcription factors upon various stimuli resulting in cell cycle arrest. Through its N-terminal domain p21Waf1 inhibits Cdk activity, whereas through the C-terminal domain it inhibits the activity of PCNA (proliferating cell nuclear antigen) to activate DNA replication. Cytosolic location of p21 counteracts its inhibitory activities.
Pathways p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) antibody (ABIN94444) Immunohistochemistry staining of colon carcinoma (paraffin-embedded sections) with an...
Image no. 2 for anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) antibody (ABIN94444) Immunofluorescence staining of p21 in human primary fibroblasts using anti-p21 (; gre...
Product cited in: Kolár, Murray, Scott, Harrison, Vojt?sek, Dusek: "Relation of Bcl-2 expression to androgen receptor, p21WAF1/CIP1, and cyclin D1 status in prostate cancer." in: Molecular pathology : MP, Vol. 53, Issue 1, pp. 15-8, 2000 (PubMed).