p21 antibody

Catalog No. ABIN94444

This Mouse Monoclonal antibody specifically detects p21 in ELISA, IHC (p) and ICC. It exhibits reactivity toward Human and Non-Human Primate. It has been mentioned in 2+ publications

Quick Overview for p21 antibody (ABIN94444)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This p21 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Clone: WA-1

Product Details anti-p21 Antibody

Purpose: Anti-p21Waf1 Purified

Cross-Reactivity (Details): Human, Non-Human Primates

Characteristics: The antibody WA-1 reacts with p21 protein (Waf1, Cip1, SDI 1, intracellular antigen), a 21 kDa tumour suppressor, which inhibits the activity of cyclin-dependent family kinases and of proliferating cell nuclear antigen.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human p21 protein

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Positive tissue: colon carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-p21 antibody (ABIN94444)

Palumbo, Gogliettino, Cocca, Iannitti, Sandomenico, Ruvo, Balestrieri, Rossi, Palmieri: "APEH Inhibition Affects Osteosarcoma Cell Viability via Downregulation of the Proteasome." in: International journal of molecular sciences, Vol. 17, Issue 10, (2017) (PubMed).

Kolár, Murray, Scott, Harrison, Vojt?sek, Dusek: "Relation of Bcl-2 expression to androgen receptor, p21WAF1/CIP1, and cyclin D1 status in prostate cancer." in: Molecular pathology : MP, Vol. 53, Issue 1, pp. 15-8, (2000) (PubMed).

Target Details for p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Alternative Name: p21Waf1

Background: Cyclin dependent kinase inhibitor 1A,The tumour suppressor p21Waf1 (wild-type p53-activated fragment 1, also known as Cip1, Cdk interacting protein, or SDI 1) is a cyclin-dependent kinase (Cdk) inhibitor, which is expressed by involvement of p53, Egr-1, AP2, STATs or other transcription factors upon various stimuli resulting in cell cycle arrest. Through its N-terminal domain p21Waf1 inhibits Cdk activity, whereas through the C-terminal domain it inhibits the activity of PCNA (proliferating cell nuclear antigen) to activate DNA replication. Cytosolic location of p21 counteracts its inhibitory activities.,CDKN1A, CIP1, CAP20

Gene ID: 1026

UniProt: P38936

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy