MUC5AC antibody

Catalog No. ABIN966607

This Mouse Monoclonal antibody specifically detects MUC5AC in IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Monkey, Cat, Rabbit, Chicken and Cow.It has been mentioned in 1 publication

Quick Overview for MUC5AC antibody (ABIN966607)

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reactivity: Human, Mouse, Rat, Monkey, Cat, Rabbit, Chicken, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC5AC antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B442(1-13M1)

Product Details anti-MUC5AC Antibody

Specificity: This antibody reacts with the peptide core of gastric mucin (MUC5AC).

Purification: Concentrated.

Immunogen: Human ovarian cyst mucin

Isotype: IgG1

Application Details

Application Notes: IHC: 1:50-1:100
ELISA 1:500-1:2000 (Not purified, cannot be used to coat ELISA plates)
Staining Procedure: This antibody can be used on frozen or formalin-fixed, paraffin-embedded tissue sections. If using on formalin tissue a protease treatment is recommended. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Comment:

Cellular Localization: Cytoplasmic and cell surface.
Recommended Positive Control: Colon or Stomach

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-MUC5AC antibody (ABIN966607)

Guyonnet Duperat, Audie, Debailleul, Laine, Buisine, Galiegue-Zouitina, Pigny, Degand, Aubert, Porchet: "Characterization of the human mucin gene MUC5AC: a consensus cysteine-rich domain for 11p15 mucin genes?" in: The Biochemical journal, Vol. 305 ( Pt 1), pp. 211-9, (1995) (PubMed).

Target Details for MUC5AC

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Alternative Name: MUC5AC

Background: Peptide core of gastric mucin M1, epitope destroyed by beta mercaptoethanol and proteases, but not with periodate(1).

Molecular Weight: >1000kDa.

Gene ID: 4586