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CD90 (THY1) antibody

Antigen

CD90 (THY1)
Synonyms CD90, FLJ33325
Clonality Monoclonal
Host
Reactivity
Alternatives

Mouse (Murine)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro))
2 references available
Catalog no. ABIN967178
Quantity 0.5 ml  (0.3 mg/ml.)  (Variants)
Price 130.63 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Thy-1
Gene ID 7070
Immunogen EL-4 mouse T cell lymphoma cells.
Isotype IgG2b kappa
Specificity This antibody reacts with mouse Thy-1, a well characterized cell surface glycoprotein with a broad tissue distribution. Thy-1 is present on mouse brain, thymocytes, peripheral T-lymphocytes and some intraepithelial Tlymphocytes. This antigen is likely to play a role in the development and function of the nervous system.
Molecular Weight 25 kDa
Comments

Cellular Localization: Cell membrane.
Recommended Positive Control: Mouse thymus

Application Details
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Application Notes Immunohistochemistry: (Aceone-fixed frozen) 1:50-1:100
Flow: 1:100-1:300 (Not tested in our lab.)
IF -1:50-1:200
Staining Procedure: This antibody can be used on frozen tissue sections only. The antibody may be used at a dilution 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.
Concentration 0.3 mg/ml.
Purification Concentrated.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Preservative 0.05% Sodium Azide
Storage Store at 2-8° C for short term, freeze under -20°C for long term storage.
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Williams, Gagnon: "Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin." in: Science (New York, N.Y.), Vol. 216, Issue 4547, pp. 696-703, 1982 (PubMed).

Beissert, He, Hueber et al.: "Impaired cutaneous immune responses in Thy-1-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 10, pp. 5296-302, 1998 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "CD90 (THY1)", type "Antibodies"
Hosts Rat (158), Mouse (155), Rabbit (33), Goat (2)
Reactivities Mouse (Murine) (230), Human (88), Rat (Rattus) (84), Dog (Canine) (27), Pig (Porcine) (24), Horse (Equine) (18), Sheep (Ovine) (18), Guinea Pig (14), Rabbit (14), Monkey (10), Primate (5)
Applications Flow Cytometry (FACS) (285), Western Blotting (WB) (51), Immunoprecipitation (IP) (50), Immunohistochemistry (Fixed) (IHC (fx)) (49), Immunohistochemistry (Frozen Sections) (IHC (fro)) (38), Cytotoxicity Test (CyTox) (28), Immunofluorescence (IF) (26), Immunocytochemistry (ICC) (25), ELISA (12), Immunohistochemistry (IHC) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Functional Studies (Func) (6), Blocking Antibody (Inhibition) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1)
Conjugates FITC (54), Biotin (48), PE (40), APC (19), RPE (8), Cy5,PE (7), Cy5.5 (4), Cy7,PE (3), SPRD (3), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes N-Term (2)