Transferrin Receptor antibody

Catalog No. ABIN967201

Product Details anti-Transferrin Receptor Antibody

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody reacts with the transferrin receptor, a 180-190 kD transmembrane glycoprotein which exists as a 95 kD homodimer with interchain disulfide bond. The specificity of these antibodies, as demonstrated by immunoprecipitation, are equivalent to OKT9, B3/25 and BerT9.

Purification: Purified.

Immunogen: Cell line KG1.

Clone: B349(DF1513)

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescense 10-20 micrograms/ml.
Flow cytometry: 1 micrograms/10^6 cells.
Immunohistochemistry: (Acetone-fixed frozen) 5-15 micrograms/mL using avidin-biotin system. This antibody is an indicator of proliferation activity. It also has prognostic significance when typing tumors, such as leukemias and lymphomas.
Staining Procedure: This antibody can be used on frozen tissue sections only in IHC.

Comment:

Cellular Localization: Cell membrane.
Recommended Positive Control: Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 0.2 mg Immunoglobulin in cell culture supernatant, 50 mM Sodium Borate, 150 mM Sodium Chloride, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-Transferrin Receptor antibody (ABIN967201)

Friden, Walus, Musso, Taylor, Malfroy, Starzyk: "Anti-transferrin receptor antibody and antibody-drug conjugates cross the blood-brain barrier." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 11, pp. 4771-5, (1991) (PubMed).

Jing, Spencer, Miller, Hopkins, Trowbridge: "Role of the human transferrin receptor cytoplasmic domain in endocytosis: localization of a specific signal sequence for internalization." in: The Journal of cell biology, Vol. 110, Issue 2, pp. 283-94, (1990) (PubMed).

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: Transferrin Receptor,CD71 (TFRC Products)

Molecular Weight: ~9095 kDa

Gene ID: 7037

Pathways: Transition Metal Ion Homeostasis