Ubiquitin antibody

Catalog No. ABIN967230

The Rabbit Polyclonal anti-Ubiquitin antibody (ABIN967230) specifically detects Ubiquitin in IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for Ubiquitin antibody (ABIN967230)

Target: Ubiquitin (Ub)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Ubiquitin Antibody

Specificity: This antibody reacts with ubiquitin, a 8.5 kD polypeptide.

Characteristics: Total Ig approx. 8 mg/ml
Spec. Ig. approx. 0.2-0.3 mg/ml.

Purification: Concentrated.

Immunogen: Ubiquitin purified from bovine erythrocytes and conjugated to phycoerythrin-R.

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100.
Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.

Comment:

Cellular Localization: Nuclear, cytoplasmic and cell membrane.
Recommended Positive Control: Normal Human Brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 8 mg/ml

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Molecular Weight: 8.5 kDa

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway