Vasodilator-Stimulated phosphoprotein (VASP) antibody

Antigen: Vasodilator-Stimulated phosphoprotein (VASP)

Synonyms: AA107290, vasp, MGC80889, VASP, MGC127789, wu:fb23b04, wu:fk84g05, zgc:110347, si:dkey-113g17.1, si:ch211-202c21.1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN967240

Additional Information

Alternative name: VASP

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human VASP around the phosphorylation site of serine 238 (K-V-SP-K-Q).

Specificity: VASP (Ab-238) antibody detects endogenous levels of total VASP protein.

Application Details

Application Notes: WB: 1:500~1:1000.

Concentration: 100ug/100ul.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: 0.02% Sodium Azide

Storage: Store at -20°C/1 year.

Restrictions: For Research Use only

Publications

Product: Butt, Abel, Krieger et al.: "cAMP- and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human platelets." in: The Journal of biological chemistry, Vol. 269, Issue 20, pp. 14509-17, 1994 (PubMed).

Smolenski, Bachmann, Reinhard et al.: "Analysis and regulation of vasodilator-stimulated phosphoprotein serine 239 phosphorylation in vitro and in intact cells using a phosphospecific monoclonal antibody." in: The Journal of biological chemistry, Vol. 273, Issue 32, pp. 20029-35, 1998 (PubMed).

Zhuang, Nguyen, Chen et al.: "Vasodilator-stimulated phosphoprotein activation of serum-response element-dependent transcription occurs downstream of RhoA and is inhibited by cGMP-dependent protein kinase phosphorylation." in: The Journal of biological chemistry, Vol. 279, Issue 11, pp. 10397-407, 2004 (PubMed).

Jensen, Selheim, Døskeland et al.: "Protein kinase A mediates inhibition of the thrombin-induced platelet shape change by nitric oxide." in: Blood, Vol. 104, Issue 9, pp. 2775-82, 2004 (PubMed).

Wang, Lu, Jin et al.: "Presynaptic and postsynaptic roles of NO, cGK, and RhoA in long-lasting potentiation and aggregation of synaptic proteins." in: Neuron, Vol. 45, Issue 3, pp. 389-403, 2005 (PubMed).