ZAP70 antibody

Catalog No. ABIN967271

The Rabbit Polyclonal anti-ZAP70 antibody has been validated for IHC. It is suitable to detect ZAP70 in samples from Human, Mouse and Rat. There are 6+ publications available.

Quick Overview for ZAP70 antibody (ABIN967271)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-ZAP70 Antibody

Specificity: Zap-70 (Ab-319) antibody detects endogenous levels of total Zap-70 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Zap-70 around the phosphorylation site of tyrosine 319 (S-P-YP-S-D).

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-ZAP70 antibody (ABIN967271)

Adjali, Marodon, Steinberg, Mongellaz, Thomas-Vaslin, Jacquet, Taylor, Klatzmann: "In vivo correction of ZAP-70 immunodeficiency by intrathymic gene transfer." in: The Journal of clinical investigation, Vol. 115, Issue 8, pp. 2287-95, (2005) (PubMed).

Salomon, Ficarro, Brill, Brinker, Phung, Ericson, Sauer, Brock, Horn, Schultz, Peters: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, (2003) (PubMed).

Ku, Yablonski, Manser, Lim, Weiss: "A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT." in: The EMBO journal, Vol. 20, Issue 3, pp. 457-65, (2001) (PubMed).

Tang, Sawasdikosol, Chang, Burakoff: "SLAP, a dimeric adapter protein, plays a functional role in T cell receptor signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 17, pp. 9775-80, (1999) (PubMed).

Williams, Irvin, Sutor, Chini, Yacyshyn, Bubeck Wardenburg, Dalton, Chan, Abraham: "Phosphorylation of Tyr319 in ZAP-70 is required for T-cell antigen receptor-dependent phospholipase C-gamma1 and Ras activation." in: The EMBO journal, Vol. 18, Issue 7, pp. 1832-44, (1999) (PubMed).

Zhao, Weiss: "Enhancement of lymphocyte responsiveness by a gain-of-function mutation of ZAP-70." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6765-74, (1997) (PubMed).

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: Zap-70

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway