Interleukin 1 Receptor, Type I (IL1R1) antibody

Details for Product No. ABIN967343
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Antigen
Synonyms CD121A, D2S1473, IL-1R-alpha, IL1R, IL1RA, P80, CD121a, CD121b, IL-iR, Il1r-1
Reactivity
Human
(63), (29), (13)
Host
Mouse
(55), (21), (17), (10), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (2), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(61), (60), (20), (16), (15), (14), (14), (10), (8), (6), (6), (3), (2), (2), (2), (2)
Pubmed 5 references available
Quantity 0.5 mg
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Catalog No. ABIN967343
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Clone HIL1R-M1
Isotype IgG1, kappa
Characteristics 1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD121a
Background The HIL1R-M1 antibody reacts with recombinant and natural soluble CD121a which is also known as human IL-1 receptor type I proteins. Human IL-1 receptor type I serves as the ligand-binding chain of the IL-1 heterodimer complex. When produced by proteolytic cleavage, soluble forms of the IL-1R type I are found the circulation of healthy individuals and in excessive levels during inflammatory diseases. The human IL-1 receptor type I is also expressed predominantly by T lymphocytes, fibroblasts, and endothelial cells.
Synonyms: IL-1 Receptor Type 1
Application Notes ELISA Capture: The purified HIL1R-M1 antibody (ABIN967343) is useful as a capture antibody for a sandwich ELISA for measuring human IL-1 receptor I protein levels. Purified HIL1R-M1 antibody can be paired with a biotinylated detecting antibody, with recombinant soluble human IL-1 receptor type I as the standard. Purified HIL1R-M1 antibody should be titrated between 1 - 4 µg/ml to determine its optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of recombinant human IL-1 receptor type I ranging from 3000 to 20 pg/ml are recommended for inclusion in each ELISA plate.
Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human cytokines: sCD14, CD40, CD40L, IL-1Ra, IL-1RII, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, sIL-4R, IL-5, IL-6, IL-6R, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, TNF, LT-alpha, sTNFRI, sTNFRII, IFNgamma, TRAIL, GM-CSF, TGFbeta, Trx Western Blot: The purified HIL1R-M1.1 antibody (ABIN967343) has been reported to be useful for Western blotting, however, the antibody is not routinely tested for this application.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: Sims: "CD121a." in: Journal of biological regulators and homeostatic agents, Vol. 14, Issue 2, pp. 133-5, 2000 (PubMed).

General Sims, Giri, Dower: "The two interleukin-1 receptors play different roles in IL-1 actions." in: Clinical immunology and immunopathology, Vol. 72, Issue 1, pp. 9-14, 1994 (PubMed).

Sims, Painter, Gow: "Genomic organization of the type I and type II IL-1 receptors." in: Cytokine, Vol. 7, Issue 6, pp. 483-90, 1996 (PubMed).

Bernstein, Fay, Frankel et al.: "A phase I study of recombinant human soluble interleukin-1 receptor (rhu IL-1R) in patients with relapsed and refractory acute myeloid leukemia." in: Cancer chemotherapy and pharmacology, Vol. 43, Issue 2, pp. 141-4, 1999 (PubMed).

Born, Smith, Garka et al.: "Identification and characterization of two members of a novel class of the interleukin-1 receptor (IL-1R) family. Delineation of a new class of IL-1R-related proteins based on signaling." in: The Journal of biological chemistry, Vol. 275, Issue 39, pp. 29946-54, 2000 (PubMed).

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