ELISA Capture: The purified HIL1R-M1 antibody (ABIN967343) is useful as a capture antibody for a sandwich ELISA for measuring human IL-1 receptor I protein levels. Purified HIL1R-M1 antibody can be paired with a biotinylated detecting antibody, with recombinant soluble human IL-1 receptor type I as the standard. Purified HIL1R-M1 antibody should be titrated between 1 - 4 µg/ml to determine its optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of recombinant human IL-1 receptor type I ranging from 3000 to 20 pg/ml are recommended for inclusion in each ELISA plate.
Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human cytokines: sCD14, CD40, CD40L, IL-1Ra, IL-1RII, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, sIL-4R, IL-5, IL-6, IL-6R, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, TNF, LT-alpha, sTNFRI, sTNFRII, IFNgamma, TRAIL, GM-CSF, TGFbeta, Trx Western Blot: The purified HIL1R-M1.1 antibody (ABIN967343) has been reported to be useful for Western blotting, however, the antibody is not routinely tested for this application.