IL1R1 antibody (pTyr496)

Catalog No. ABIN801423

The Rabbit Polyclonal anti-IL1R1 antibody (ABIN801423) specifically detects IL1R1 in WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p) and IHC (fro). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for IL1R1 antibody (pTyr496) (ABIN801423)

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1R1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-IL1R1 Antibody

Binding Specificity: pTyr496

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human IL-1R1 around the phosphorylation site of Tyr496

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for IL1R1

Target: IL1R1 (Interleukin 1 Receptor, Type I (IL1R1))

Alternative Name: IL-1R1

Background:

Synonyms: P8, IL1R, IL1RA, CD121A, D2S1473, IL-1R-alpha, Interleukin-1 receptor type 1, IL-1R-1, IL-1RT-1, IL-1RT1, CD121 antigen-like family member A, Interleukin-1 receptor alpha, Interleukin-1 receptor type I, IL1R1, IL1RT1

Background: Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex.

Gene ID: 3554

UniProt: P14778

Pathways: NF-kappaB Signaling, Carbohydrate Homeostasis, Cancer Immune Checkpoints