SYK antibody (AA 314-339)

Catalog No. ABIN967352

The Mouse Monoclonal anti-SYK antibody has been validated for WB and FACS. It is suitable to detect SYK in samples from Human. There are 4+ publications available.

Quick Overview for SYK antibody (AA 314-339) (ABIN967352)

Target: SYK (Spleen tyrosine Kinase (SYK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SYK antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 4D10

Product Details anti-SYK Antibody

Binding Specificity: AA 314-339

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Syk peptide aa. 314-339

Isotype: IgG2a

Application Details

Application Notes: Western Blot: EB1 control lysate [50 µg (1 µg/ml)] is provided as a positive control (store lysate at -20°C).
Additional application: The FITC-conjugated format of clone 4D10 is useful in immunofluorescent staining and flow cytometric analysis.

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: SDS-PAGE buffer (62 mM Tris pH 6.8, 2 % SDS, 0.9 % b-mercaptoethanol, 0.003 % bromophenol blue, 5 % glycerol)

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-SYK antibody (ABIN967352)

Latour, Veillette: "Proximal protein tyrosine kinases in immunoreceptor signaling." in: Current opinion in immunology, Vol. 13, Issue 3, pp. 299-306, (2001) (PubMed).

Turner, Schweighoffer, Colucci, Di Santo, Tybulewicz: "Tyrosine kinase SYK: essential functions for immunoreceptor signalling." in: Immunology today, Vol. 21, Issue 3, pp. 148-54, (2000) (PubMed).

Chu, van Oers, Malissen, Harris, Elder, Weiss: "Pre-T cell receptor signals are responsible for the down-regulation of Syk protein tyrosine kinase expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2610-20, (1999) (PubMed).

Costello, Turner, Walters, Cunningham, Bauer, Downward, Tybulewicz: "Critical role for the tyrosine kinase Syk in signalling through the high affinity IgE receptor of mast cells." in: Oncogene, Vol. 13, Issue 12, pp. 2595-605, (1997) (PubMed).

Target Details for SYK

Target: SYK (Spleen tyrosine Kinase (SYK))

Alternative Name: Syk

Background: Syk is a member of the family of non-receptor type protein-tyrosine kinases and plays a crucial role in lymphocyte signaling and development. Syk is expressed in all haematopoietic cells and contributes to the signal transduction process by binding to a tyrosine-based activation motif (ITAM) of immune receptors, including Igalpha, TCRzeta, CD3epsilon, FcepsilonRIbeta, and FceRIgamma. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains thereby activating Syk and causing tyrosines in Syk to undergo auto-phosphorylation or phosphorylation. These phosphorylated sites can act as binding sites for other signaling molecules or help to regulate enzymatic activity. For example, in mast cells, Syk can activate downstream targets such as phospholipase Cgamma1 and VAV. Studies in Syk-/- mast cells identified defects in both the ERK-MAP and JNK-MAP kinase pathways. Syk migrates as 72 kDa in SDS/PAGE. (Predicted MW from SWISS-PROT is 72 kDa, Accession number P43405).

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling