CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody

Antigen: CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E)

Synonyms: T3E, TCRE, FLJ18683, CD3E, cd3e, CD3, t3e, tcre

Clonality: Monoclonal (145-2C11)

Host: Armenian Hamster

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Co-Stimulation (CoSt), Immunoprecipitation (IP), Western Blotting (WB), Cytotoxicity Test (CyTox), Blocking Antibody (Inhibition), Immunofluorescence (IF)

Catalog no.: ABIN967370

Additional Information

Alternative name: CD3e

Immunogen: H-2Kb specific cytotoxic T lymphocyte clone BM10-37

Format: Liquid

Isotype: IgG1, kappa

Clone: 145-2C11

Description: The 145-2C11 antibody reacts with the 25-kDa epsilon chain of the T-cell receptor-associated CD3 complex, which is expressed on thymocytes, mature T lymphocytes, and NK-T cells. The cytoplasmic domain of CD3e participates in the signal transduction events which activate several cellular biochemical pathways as a result of antigen recognition. Soluble 145-2C11 antibody can activate either unprimed (naive) or primed (memory/preactivated) T cells in vivo or in vitro, in the presence of Fc receptor-bearing accessory cells. In contrast, plate-bound 145-2C11 can activate T cells in the absence of accessory cells. Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's. Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death. The 145-2C11 antibody does not cross-react with rat leukocytes and it has been reported that pre-incubation of thymus cell suspensions at 37°C for 2-4 hours prior to staining enhances the ability of anti-CD3epsilon and anti-alphabeta TCR mAbs to detect the T-cell receptor on immature thymocytes. This antibody is routinely tested by flow cytometric analysis.
Synonyms: CD3epsilon chain

Characteristics: 1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs.
4. Please refer to us for technical protocols.

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4° C.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

CD3e expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with PE-conjugated anti-mouse CD4 mAb RM4-5, PE-conjugated anti-mouse CD8a mAb 53-6.7 and either purified anti-mouse CD3e mAb 145-2C11 (third panel) or isotype control (first panel), followed by staining with a FITC-conjugated anti-hamster IgG mAb cocktail.

CD3e expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with PE-conjugated anti-mouse CD4 mAb RM4-5, PE-conjugated anti-mouse CD8a mAb 53-6.7 and either purified anti-mouse CD3e mAb 145-2C11 (third panel) or isotype control (first panel), followed by staining with a FITC-conjugated anti-hamster IgG mAb cocktail.

CD3e expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with PE-conjugated anti-mouse CD4 mAb RM4-5, PE-conjugated anti-mouse CD8a mAb 53-6.7 and either purified anti-mouse CD3e mAb 145-2C11 (third panel) or isotype control (first panel), followed by staining with a FITC-conjugated anti-hamster IgG mAb cocktai.

Publications

Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, 1991 (PubMed).

Kubo, Born, Kappler et al.: "Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2736-42, 1989 (PubMed).

Portoles, Rojo, Golby et al.: "Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 12, pp. 4169-75, 1989 (PubMed).

Leo, Foo, Sachs et al.: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, 1987 (PubMed).

Isakov, Wange, Burgess et al.: "ZAP-70 binding specificity to T cell receptor tyrosine-based activation motifs: the tandem SH2 domains of ZAP-70 bind distinct tyrosine-based activation motifs with varying affinity." in: The Journal of experimental medicine, Vol. 181, Issue 1, pp. 375-80, 1995 (PubMed).

Vossen, Tibbe, Kroos et al.: "Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects." in: European journal of immunology, Vol. 25, Issue 6, pp. 1492-6, 1995 (PubMed).

Salvadori, Gansbacher, Pizzimenti et al.: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, 1994 (PubMed).

Nakano, Yamazaki, Miyatake et al.: "Specific interaction of topoisomerase II beta and the CD3 epsilon chain of the T cell receptor complex." in: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6483-9, 1996 (PubMed).

Wagner, Hagman, Linsley et al.: "Rescue of thymocytes from glucocorticoid-induced cell death mediated by CD28/CTLA-4 costimulatory interactions with B7-1/B7-2." in: The Journal of experimental medicine, Vol. 184, Issue 5, pp. 1631-8, 1996 (PubMed).

Chai, Lechler: "Immobilized anti-CD3 mAb induces anergy in murine naive and memory CD4+ T cells in vitro." in: International immunology, Vol. 9, Issue 7, pp. 935-44, 1997 (PubMed).

Product: Shinkai, Alt: "CD3 epsilon-mediated signals rescue the development of CD4+CD8+ thymocytes in RAG-2-/- mice in the absence of TCR beta chain expression." in: International immunology, Vol. 6, Issue 7, pp. 995-1001, 1994 (PubMed).

Martin, Lenardo: "Morphological, biochemical, and flow cytometric assays of apoptosis." in: Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.], Vol. Chapter 14, pp. Unit 14.13, 2008 (PubMed).

Kruisbeek, Shevach, Thornton: "Proliferative assays for T cell function." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 3, pp. Unit 3.12, 2008 (PubMed).