TER-119/Erythroid Cells antibody

Catalog No. ABIN967376

There are 2+ publications for this product available. The Rat Monoclonal anti-TER-119/Erythroid Cells antibody is suitable to detect TER-119/Erythroid Cells in samples from Mouse. It has been validated for IHC (fro), FACS, IP, WB, CyTox and IHC (p).

Quick Overview for TER-119/Erythroid Cells antibody (ABIN967376)

Target: TER-119/Erythroid Cells

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Cytotoxicity Test (CyTox), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TER-119

Product Details

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Fetal Liver

Isotype: IgG2b kappa

Application Details

Application Notes: For IHC, we recommend the use of purified TER-119 mAb in our special formulation for immunohistochemistry.
Caution: Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C.

References

Kina, Ikuta, Takayama, Wada, Majumdar, Weissman, Katsura: "The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage." in: British journal of haematology, Vol. 109, Issue 2, pp. 280-7, (2000) (PubMed).

Ikuta, Kina, MacNeil, Uchida, Peault, Chien, Weissman: "A developmental switch in thymic lymphocyte maturation potential occurs at the level of hematopoietic stem cells." in: Cell, Vol. 62, Issue 5, pp. 863-74, (1990) (PubMed).

Target Details

Target: TER-119/Erythroid Cells

Background: The TER-119 antibody reacts with a 52 kDa molecule associated with glycophorin A on cells of the erythroid lineage in embryonic yolk sac, fetal liver, newborn liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs. The TER-119 antigen is expressed on erythroid cells from pro-erythroblast through mature erythrocyte stages, but not on cells with BFU-E or CFU-E activities. The TER-119 epitope is not detected on hematopoietic stem cells, lymphoid cells, myeloid cells, or erythroleukemia lines. The TER-119 mAb is a component of the lineage cocktail used in studies of hematopoietic progenitors to detect, or deplete cells committed to the hematopoietic lineages. This antibody is routinely tested by flow cytometric analysis.
Synonyms: Ly-76