Interferon gamma (IFNG) antibody

Details for Product No. ABIN967461
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Synonyms IFNG2, IFN-g, Ifg, IFN-G, IFN-gamma, IFNG, IFG, IFI
Mouse (Murine)
(473), (237), (117), (49), (20), (18), (17), (14), (10), (7), (6), (6), (4), (4), (3), (3), (1), (1)
(400), (297), (154), (31), (10), (6), (4), (2), (1)
Clonality (Clone)
Monoclonal ()
(119), (74), (51), (28), (25), (9), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
ELISA, Neutralization (Neut), Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
(520), (478), (248), (124), (109), (106), (64), (62), (47), (41), (34), (30), (29), (27), (23), (17), (10), (9), (9), (7), (5), (5), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Catalog No. ABIN967461
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Immunogen Mouse IFN-gamma
Clone XMG1-2
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name IFN-gamma
Background The XMG1.2 antibody reacts with mouse interferon-gamma (IFN-gamma) protein. IFN-gamma is a pleiotropic cytokine, of approximately 15-17 kDa, involved in the regulation of the immune response. It plays an important role in activation, growth, and differentiation of T and B lymphocytes, macrophages, NK cells and other non-hematopoietic cell types. IFN-gamma production is associated with the Th-1 differentiation. This is a neutralizing antibody
Research Area Cytokines
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Interferon gamma (IFNG) antibody Expression of IFN-gamma by stimulated CD8+ and CD8-BALB/c spleen cells. Splenocytes from 6 month old BALB/c mice were cultured for 3 days in the presence of SEB (2 µg/ml, Sigma), then restimulated for 5 hr with hamster anti-mouse CD3 (2 µg/ml, 145-2C11) and hamster anti-mouse CD28 (2 µg/ml, 37.51) antibodies in the presence of 2 µg GolgiStop™ (aka Monensin). The splenocytes were harvested, stained with 0.06 µg of FITC-conjugated rat anti-mouse CD48 (FITC-53-6.7), fixed, permeabilized, and subsequently stained with 0.06 µg of PE-conjugated rat anti-mouse IFN-gamma antibody (PE-XMG1.2) by using Pharmingen's staining protocol (first panel). To demonstrate specificity of staining, the binding by the PE-XMG1.2 antibody was blocked by preincubation of the fixed/permeabilized cells with unlabeled XMG1.2 antibody (5.0 µg, second panel) prior to staining. The quadrant markers for the bivariate dot plots were set based on the autofluorescence controls and verified using the recombinant cytokine blocking and unlabeled antibody blocking specificity controls.
anti-Interferon gamma (IFNG) antibody (2) See first image description
General Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).

Sander, Höidén, Andersson et al.: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994 (PubMed).

Cherwinski, Schumacher, Brown et al.: "Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies." in: The Journal of experimental medicine, Vol. 166, Issue 5, pp. 1229-44, 1987 (PubMed).

Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992 (PubMed).

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