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IFNG antibody (Interferon gamma)

Details for Product anti-IFNG Antibody No. ABIN967461, Supplier: Log in to see
Antigen
  • IFNG2
  • IFN-g
  • Ifg
  • IFN-G
  • IFN-gamma
  • IFNG
  • IFG
  • IFI
Alternatives
anti-Mouse (Murine) IFNG antibody for Enzyme Immunoassay
Reactivity
Mouse (Murine)
613
263
145
57
35
25
20
20
13
13
12
11
9
9
9
9
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6
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6
6
5
2
1
1
1
Host
Rat
527
389
166
30
8
7
4
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This IFNG antibody is un-conjugated
156
112
61
46
35
12
10
10
9
6
5
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4
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3
3
3
3
3
3
2
2
2
2
2
2
1
1
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Application
Blocking Antibody (Inhibition), Blocking Reagent (BR), Neutralization (Neut), ELISA, Western Blotting (WB)
623
550
350
123
116
114
69
60
48
40
38
37
37
32
29
17
14
9
8
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7
7
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4
4
4
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2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Brand BD Pharmingen™
Immunogen Mouse IFN-gamma
Clone XMG1-2
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name IFN-gamma (IFNG Antibody Abstract)
Background The XMG1.2 antibody reacts with mouse interferon-gamma (IFN-gamma) protein. IFN-gamma is a pleiotropic cytokine, of approximately 15-17 kDa, involved in the regulation of the immune response. It plays an important role in activation, growth, and differentiation of T and B lymphocytes, macrophages, NK cells and other non-hematopoietic cell types. IFN-gamma production is associated with the Th-1 differentiation. This is a neutralizing antibody
Research Area Cytokines
Pathways Interferon-gamma Pathway
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
 image for anti-IFNG antibody (Interferon gamma) (ABIN967461) Expression of IFN-gamma by stimulated CD8+ and CD8-BALB/c spleen cells. Splenocytes f...
 image for anti-IFNG antibody (Interferon gamma) (ABIN967461) See first image description
Product cited in: Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).

Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992 (PubMed).

Background publications Sander, Höidén, Andersson et al.: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994 (PubMed).

Cherwinski, Schumacher, Brown et al.: "Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal ..." in: The Journal of experimental medicine, Vol. 166, Issue 5, pp. 1229-44, 1987 (PubMed).