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CD47 antibody (CD47)

Details for Product anti-CD47 Antibody No. ABIN967480, Supplier: Log in to see
Antigen
  • 9130415E20Rik
  • AA407862
  • AI848868
  • AW108519
  • B430305P08Rik
  • IAP
  • Itgp
  • MER6
  • OA3
  • CD47/IAP
Reactivity
Mouse (Murine)
150
53
47
7
4
3
3
3
2
Host
Rat
112
70
22
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD47 antibody is un-conjugated
19
18
10
8
5
4
4
3
2
2
1
1
1
1
1
1
1
1
Application
Fluorescence Microscopy (FM), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
121
90
49
34
31
22
20
17
11
9
6
6
5
5
4
4
2
1
1
Supplier
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Brand BD Pharmingen™
Immunogen Not reported
Clone Miap301
Isotype IgG2a, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD47 (IAP) (CD47 Antibody Abstract)
Background The miap301 antibody reacts with the extracellular domain of CD47, also known as Integrin-Associated Protein (IAP).1 CD47 is a 50-kDa plasma membrane protein with an extracellular immunoglobulin variable region-like domain, a multiple membrane-spanning segment, and a short C-terminal cytoplasmic tail. IAP is often physically associated with beta3 integrins, and it may act as a transducer element in activation mediated by these integrins.CD47 is expressed by a wide variety of cell types, including some cells lacking integrins, such as erythrocytes. The cytoplasmic tail region is expressed as alternatively spliced forms, which are differentially expressed in diverse tissues, suggesting that IAP may have distinct functions in various tissues. CD47 and its ligand, CD172a, are proposed to mediate bi-directional signaling to modify neural synaptic activity and regulate the phagocytic activities of macrophages.
Synonyms: IAP
Pathways
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Product cited in: Oldenborg, Zheleznyak, Fang et al.: "Role of CD47 as a marker of self on red blood cells." in: Science (New York, N.Y.), Vol. 288, Issue 5473, pp. 2051-4, 2000 (PubMed).

Gresham, Dale, Potter et al.: "Negative regulation of phagocytosis in murine macrophages by the Src kinase family member, Fgr." in: The Journal of experimental medicine, Vol. 191, Issue 3, pp. 515-28, 2000 (PubMed).

Mi, Jiang, Weng et al.: "Expression of a synapse-associated membrane protein, P84/SHPS-1, and its ligand, IAP/CD47, in mouse retina." in: The Journal of comparative neurology, Vol. 416, Issue 3, pp. 335-44, 2000 (PubMed).

Jiang, Lagenaur, Narayanan: "Integrin-associated protein is a ligand for the P84 neural adhesion molecule." in: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 559-62, 1999 (PubMed).

Lindberg, Bullard, Caver et al.: "Decreased resistance to bacterial infection and granulocyte defects in IAP-deficient mice." in: Science (New York, N.Y.), Vol. 274, Issue 5288, pp. 795-8, 1996 (PubMed).

Reinhold, Lindberg, Plas et al.: "In vivo expression of alternatively spliced forms of integrin-associated protein (CD47)." in: Journal of cell science, Vol. 108 ( Pt 11), pp. 3419-25, 1996 (PubMed).

Background publications Lindberg, Gresham, Schwarz et al.: "Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane-spanning domains implicated in alpha v beta 3-dependent ligand binding." in: The Journal of cell biology, Vol. 123, Issue 2, pp. 485-96, 1993 (PubMed).