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CD47 antibody (CD47) Primary Antibody

CD47 Reactivity: Human, Mouse FACS, IF, IHC, StM Host: Mouse Monoclonal B6H12-2
Catalog No. ABIN6941211
$508.20
Plus shipping costs $45.00
100 μg ABIN6941211
100 μg ABIN6941211
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  • Target
    Reactivity
    Human, Mouse
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    This CD47 antibody is un-conjugated
    Application
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)
    Specificity
    This antibody reacts with Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
    Purification
    Purified by Protein A/G
    Immunogen
    Intact CD47 Purified from placenta
    Clone
    B6H12-2
    Isotype
    IgG1, kappa
  • Application Notes

    Positive Control: MCF-7, HUVEC, OVCA3 cells, Placenta, Brain and Ovarian Tumors.

    Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    Alternative Name
    CD47 (CD47 Antibody Abstract)
    Molecular Weight
    ~50kDa
    Gene ID
    961
    UniProt
    Q08722
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