CD47 antibody (CD47)

Catalog No. ABIN94154

Product Details anti-CD47 Antibody

Target:

CD47

Reactivity: Human, Non-Human Primate, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD47 antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The antibody MEM-122 reacts with CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor, immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.
HLDA VI, WS Code AS A051

Cross-Reactivity (Details): Non-Human Primates, Human, Porcine

Purification: Purified by precipitation and chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: COS-7 (African green monkey) cells

Clone: MEM-122

Isotype: IgM

Target Details for CD47

Target:

CD47

Alternative Name: CD47 (CD47 Antibody Abstract)

Background: CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages, this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-CD47 antibody (ABIN94154)

Smith, Patel, Seatter, Deehan, Brown, Brooke, Goodridge, Howard, Rigley, Harnett, Harnett: "A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes." in: Blood, Vol. 102, Issue 7, pp. 2532-40, 2003 (PubMed).