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CD47 antibody (CD47) Primary Antibody

CD47 Reactivity: Human, Non-Human Primate, Pig IHC (fro) Host: Mouse Monoclonal MEM-122
Pubmed (1)
Catalog No. ABIN94154
$350.66
Plus shipping costs $45.00
0.1 mg
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  • Target
    Reactivity
    Human, Non-Human Primate, Pig
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    This CD47 antibody is un-conjugated
    Application
    Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    The antibody MEM-122 reacts with CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor, immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.
    HLDA VI, WS Code AS A051
    Cross-Reactivity (Details)
    Non-Human Primates, Human, Porcine
    Purification
    Purified by precipitation and chromatography
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    COS-7 (African green monkey) cells
    Clone
    MEM-122
    Isotype
    IgM
  • Target
    Alternative Name
    CD47 (CD47 Antibody Abstract)
    Background
    CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages, this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
  • Smith, Patel, Seatter, Deehan, Brown, Brooke, Goodridge, Howard, Rigley, Harnett, Harnett: "A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes." in: Blood, Vol. 102, Issue 7, pp. 2532-40, 2003 (PubMed).

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