CD47 antibody (CD47)

Details for Product anti-CD47 Antibody No. ABIN94154
Antigen
Reactivity
Human, Non-Human Primate, Pig (Porcine)
259
96
48
11
8
6
4
4
2
2
1
1
1
Host
Mouse
180
98
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD47 antibody is un-conjugated
20
14
12
7
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro))
193
106
87
59
54
49
35
27
22
13
11
10
7
5
5
4
3
2
2
1
1
Options
Immunogen COS-7 (African green monkey) cells
Clone MEM-122
Isotype IgM
Specificity The antibody MEM-122 reacts with CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor, immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.
HLDA VI, WS Code AS A051
Cross-Reactivity (Details) Non-Human Primates, Human, Porcine
Purification Purified by precipitation and chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD47 (CD47 Antibody Abstract)
Background CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages, this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD47 (CD47) antibody (ABIN94154) Surface staining of lysed and washed porcine peripheral blood with purified anti-CD47...
Product cited in: Smith, Patel, Seatter, Deehan, Brown, Brooke, Goodridge, Howard, Rigley, Harnett, Harnett: "A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes." in: Blood, Vol. 102, Issue 7, pp. 2532-40, 2003 (PubMed).