Western blot analysis of p21 in human and mouse. MCF-7 human (lanes 1 and 2) and EL4 mouse (lanes 3 and 4) lysates were incubated with clone SXM30 (lanes 1 and 3) or a mouse IgG1 isotype (negative) control (lanes 2 and 4). The mouse (Ms) homolog of human (Hu) p21 is slightly smaller than that human p21.
Anti-human p21, ABIN967528. Formalin-fixed, paraffin-embedded tissue section of human colon was stained with anti-p21 (clone SXM30), followed by a DAB chromogen detection system, then counter stained with Hematoxylin. Antigen retrieval using Retrievagen A Buffer Set is required. Positive staining can be identified as the intensed brown labeling.