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GTPase Kras antibody

Details for Product No. ABIN967528
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Antigen
Synonyms
Kras2, c-Ki-ras, p21, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2, SIIR, pp21, 0610011M09Rik, BC011290, P21, AI929937, K-ras, Ki-ras, Kras-2, p21B, ras, wu: ... show more
Kras2, c-Ki-ras, p21, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2, SIIR, pp21, 0610011M09Rik, BC011290, P21, AI929937, K-ras, Ki-ras, Kras-2, p21B, ras, wu:fa04e08, wu:fc14b12, wu:fc23g10, wu:fj89d12, zgc:85725 show less
Reactivity
Human
(84), (39), (39), (13), (2), (1), (1), (1)
Host
Mouse
(73), (18), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(70), (38), (24), (20), (11), (7), (6), (4), (2), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN967528
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Immunogen Human p21
Clone SXM30
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name p21
Background Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. The activity of these complexes is modulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including, p16, p21, p27, and others. These proteins, referred to as inhibitors of Cdk activity (CdkIs), bind to cyclins, cdks or their complexes. p21, also known as senescent cell-derived inhibitor 1 (Sdi1), wild-type p53-activated fragment 1 (Waf1), Cdk-interacting protein 1 (Cip1), and p53-regulated inhibitor of Cdks (Pic1) inhibits cyclin D-cdk4, cyclin E-cdk3, cyclin A-cdk2, and cyclin A-cdk1. p21 can also inhibit cell cycle progression by binding to PCNA and blocking DNA replication. p21 has also been shown to be a component of active cyclin-cdk complexes, suggesting that p21-containing complexes may shift between active and inactive states through changes in p21 content. Active, p21-containing complexes appear to contain one p21 molecule, whereas inactive compexes contain multiple p21 molecules. The expression of p21 can be induced in response to number of signals, including transcriptional upregulation by the tumor suppressor protein, p53. Human p21 has a calculated molecular weight of 18 kDa and runs at 21 kDa in SDS-PAGE.
Clone SXM30 reacts with human, rat, and mouse p21. With regard to cross-species recognition, SXM30 has similar properties to a related p21 clone, SX118. Purified, recombinant human p21-fusion protein was used as immunogen.
Molecular Weight 21 kDa
Research Area Cell Cycle
Application Notes Clone SXM30 has been shown to be useful for immunoprecipitation (1-2 µg/1x10^6 cells), western blot analysis (1-2 µg/ml), indirect immunofluorescence microscopy of tissuecultured cells, immunohistochemistry of frozen, acetone-fixed tissue sections (1-5 µg/ml), and paraffin-embedded tissue sections requiring antigen retrieval (20 µg/ml). These applications are similar to that described for a related p21 clone, SX118.3 MCF-7 human breast carcinoma cells (ATCC HTB-22), HL-60 human leukemia cells (ATCC CCL-240), and EL4 mouse lymphoma cells (ATCC TIB-39) are suggested as positive controls. In immunohistochemistry, SX118 staining is primarily nuclear. Tonsil and colon are suggested as positive controls for tissue staining. In order to obtainn optimal indirect immunohistochemical staining, clone SXM30 should be titrated at 10-20 ug/ml and visualized via a three-step staining procedure in combination with a biotinylated polyclonal anti-mouse Ig (multiple absorbson) as the secondary antibody and Streptavidin-HRP together with the DAB chromogen detection system.
Comment

Related Products: ABIN967389, ABIN967527

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-GTPase Kras antibody Western blot analysis of p21 in human and mouse. MCF-7 human (lanes 1 and 2) and EL4 mouse (lanes 3 and 4) lysates were incubated with clone SXM30 (lanes 1 and 3) or a mouse IgG1 isotype (negative) control (lanes 2 and 4). The mouse (Ms) homolog of human (Hu) p21 is slightly smaller than that human p21.
anti-GTPase Kras antibody (2) Anti-human p21, ABIN967528. Formalin-fixed, paraffin-embedded tissue section of human colon was stained with anti-p21 (clone SXM30), followed by a DAB chromogen detection system, then counter stained with Hematoxylin. Antigen retrieval using Retrievagen A Buffer Set is required. Positive staining can be identified as the intensed brown labeling.
anti-GTPase Kras antibody (3) anti-GTPase Kras antibody (Image 3)
Product cited in: Graña, Reddy: "Cell cycle control in mammalian cells: role of cyclins, cyclin dependent kinases (CDKs), growth suppressor genes and cyclin-dependent kinase inhibitors (CKIs)." in: Oncogene, Vol. 11, Issue 2, pp. 211-9, 1995 (PubMed).

Fredersdorf, Milne, Hall et al.: "Characterization of a panel of novel anti-p21Waf1/Cip1 monoclonal antibodies and immunochemical analysis of p21Waf1/Cip1 expression in normal human tissues." in: The American journal of pathology, Vol. 148, Issue 3, pp. 825-35, 1997 (PubMed).

Levine: "p53, the cellular gatekeeper for growth and division." in: Cell, Vol. 88, Issue 3, pp. 323-31, 1997 (PubMed).

Validation Images
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