Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) antibody

Details for Product No. ABIN967534
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Antigen
Synonyms TX, ICH-2, Mih1/TX, ICEREL-II, ICE(rel)II, Casp11, Caspl, ich-3, CASP4
Reactivity
Human
(107), (62), (33), (12), (7), (2)
Host
Mouse
(121), (12), (8), (5), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(8), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(129), (57), (44), (34), (19), (18), (15), (6), (3), (2), (1), (1)
Pubmed 2 references available
Catalog no. ABIN967534
Quantity 0.1 mg
Price
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Immunogen Recombinant Human Caspase-4
Clone B25-1
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Caspase-4
Background Caspase-4 (ICErelII, TX, ICH-2) is a 43 kDa cytosolic protein with homology to the ICE/Ced-3 family of cysteine proteases. Functional similarity with ICE proteases include the ability of Caspase-4 to induce apoptosis when overexpressed. Caspase-4 exhibits protease activity, cleaving both itself and the p30 ICE/caspase-1 proenzyme. However, unlike ICE/caspase-1, caspase-4 displays no IL-1beta processing activity. Caspase-4 mRNA is expressed in most adult human tissues, suggesting a broad tissue distribution. The B25-1 antibody recognizes an ~43 kDa band corresponding to human Caspase-4 (TX). A recombinant human Caspase-4 protein fragment corresponding to amino acids 334-377 was used as immunogen.
Synonyms: TX, Ich-2
Molecular Weight 43 kDa
Research Area Apoptosis/Necrosis
Application Notes Applications include western blot analysis (1-2 µg/ml). Jurkat T cells (ATCC TIB- 152) and 293 adenovirus-transformed human kidney cells (ATCC CRL-1573) are suggested as positive controls.
Comment

Related Products: ABIN967389, ABIN968537

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Storage 4 °C
Supplier Images
anti-Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) antibody Western blot analysis of Caspase-4 (TX). Lysates from 293 adenovirus-transformed human kidney cells were probed with anti-Caspase-4 (clone B25-1). The B25-1 antibody identifies Caspase-4 as an ~43 kDa band.
anti-Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) antibody (2) anti-Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) antibody (Image 2)
Product cited in: Faucheu, Diu, Chan et al.: "A novel human protease similar to the interleukin-1 beta converting enzyme induces apoptosis in transfected cells." in: The EMBO journal, Vol. 14, Issue 9, pp. 1914-22, 1995 (PubMed).

Munday, Vaillancourt, Ali et al.: "Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15870-6, 1995 (PubMed).

Hosts (121), (12), (8), (5), (5)
Reactivities (107), (62), (33), (12), (7), (2)
Applications (129), (57), (44), (34), (19), (18), (15), (6), (3), (2), (1), (1)
Conjugates (8), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (24), (12), (7), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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