Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) antibody

Antigen: Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4)

Synonyms: TX, ICH-2, Mih1/TX, ICEREL-II, ICE(rel)II, Casp11, MGC124949, CASP4, CASP13, MGC137679

Clonality: Monoclonal (B25-1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN967534

Additional Information

Alternative name: Caspase-4

Immunogen: Recombinant Human Caspase-4

Format: Liquid

Isotype: IgG1

Clone: B25-1

Description: Caspase-4 (ICErelII, TX, ICH-2) is a 43 kDa cytosolic protein with homology to the ICE/Ced-3 family of cysteine proteases. Functional similarity with ICE proteases include the ability of Caspase-4 to induce apoptosis when overexpressed. Caspase-4 exhibits protease activity, cleaving both itself and the p30 ICE/caspase-1 proenzyme. However, unlike ICE/caspase-1, caspase-4 displays no IL-1beta processing activity. Caspase-4 mRNA is expressed in most adult human tissues, suggesting a broad tissue distribution. The B25-1 antibody recognizes an ~43 kDa band corresponding to human Caspase-4 (TX). A recombinant human Caspase-4 protein fragment corresponding to amino acids 334-377 was used as immunogen.
Synonyms: TX, Ich-2

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 43 kDa

Comments:

Related Products: ABIN967389, ABIN968537

Application Details

Application Notes: Applications include western blot analysis (1-2 µg/ml). Jurkat T cells (ATCC TIB- 152) and 293 adenovirus-transformed human kidney cells (ATCC CRL-1573) are suggested as positive controls.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of Caspase-4 (TX). Lysates from 293 adenovirus-transformed human kidney cells were probed with anti-Caspase-4 (clone B25-1). The B25-1 antibody identifies Caspase-4 as an ~43 kDa band.

anti-Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) antibody (Image 2)

Publications

Product: Faucheu, Diu, Chan et al.: "A novel human protease similar to the interleukin-1 beta converting enzyme induces apoptosis in transfected cells." in: The EMBO journal, Vol. 14, Issue 9, pp. 1914-22, 1995 (PubMed).

Munday, Vaillancourt, Ali et al.: "Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15870-6, 1995 (PubMed).