TEK Tyrosine Kinase, Endothelial (TEK) (Extracellular Domain) antibody

Antigen: TEK Tyrosine Kinase, Endothelial (TEK)

Synonyms: TIE2, VMCM, TIE-2, VMCM1, CD202B, MGC139569, TEK, DKFZp469E064

Binding Site: Extracellular Domain

Clonality: Monoclonal (33)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN967565

Additional Information

Alternative name: TIE2

Immunogen: Human TIE2 Recombinant Protein

Cross-Reactivity: Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: 33

Description: The TIE receptor family is a member of the class of receptors called the receptor tyrosine kinases (RKT). RKTs are cell surface receptors which also contain a protein kinase domain in their intracellular portion and upon binding of their specific growth factor, initiate a series of downstream biological events. TIE receptors, designated Tie1 and Tie2 (Tek) have a tissue distribution which is limited primarily to cells of endothelial origin. Tie2 has been shown to be required for the normal development of vascular structures during embryogenesis. It has also been demonstrated to play a role in normal and pathological angiogenesis in adults. Tie2 has a predicted molecular weight of 126 kD, but may be observable at ~130-165 kDa. This antibody has been reported to recognize human and mouse Tie2 but not Tie1.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.

Molecular Weight: 130-165 kDa

Comments:

Related Products: ABIN967389, ABIN968537

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Organogenesis, Tyrosine Kinases

Restrictions: For Research Use only

Images

Western blot analysis for Tie2. Human endothelial cell lysates were probed with the mouse anti-Tie2 antibody at concentrations of 4 µg/mL (lane 1), 2 µg/mL (lane 2), and 1 µg/mL (lane 3). Due to glycosylation, Tie2 may be observable migrating in a range between ~130-165 kDa.

Publications

Product: Dumont, Gradwohl, Fong et al.: "Dominant-negative and targeted null mutations in the endothelial receptor tyrosine kinase, tek, reveal a critical role in vasculogenesis of the embryo." in: Genes & development, Vol. 8, Issue 16, pp. 1897-909, 1994 (PubMed).

Wong, Haroon, Werner et al.: "Tie2 expression and phosphorylation in angiogenic and quiescent adult tissues." in: Circulation research, Vol. 81, Issue 4, pp. 567-74, 1997 (PubMed).

Peters, Coogan, Berry et al.: "Expression of Tie2/Tek in breast tumour vasculature provides a new marker for evaluation of tumour angiogenesis." in: British journal of cancer, Vol. 77, Issue 1, pp. 51-6, 1998 (PubMed).

Partanen, Dumont: "Functions of Tie1 and Tie2 receptor tyrosine kinases in vascular development." in: Current topics in microbiology and immunology, Vol. 237, Issue 4, pp. 159-72, 1999 (PubMed).