Btk and Itk (pTyr551,pTyr511) antibody

Antigen: Btk and Itk

Binding Site: pTyr551,pTyr511

Clonality: Monoclonal (24a)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN967576

Additional Information

Immunogen: Phosphorylated Human Btk

Format: Liquid

Isotype: IgG1

Clone: 24a

Description: Bruton's tyrosine kinase (Btk) is a nonreceptor tyrosine kinase whose function is critical for proper B cell development and signaling. The activity of Btk is regulated by Src mediated phosphorylation of the kinase domain at tyrosine 551 (Y551). This event induces Btk kinase activity and subsequent autophosphorylation at Y223. Phosphorylated Btk then associates with the cell membrane via the interaction of the PH domain with phosphatidylinositol 3, 4, 5-triphosphate. The Tec family kinase Itk plays a critical role in signal transduction downstream of the T cell antigen receptor and has been implicated in the activation of phospholipase C-gamma1 (PLCgamma1), a key regulator of calcium mobilization and extracellular signal-regulated kinase (ERK) activation. Itk is regulated by an activating transphosphorylation event in which Y511 in the kinase domain is phosphorylated by Lck. The 24a/BTK (Y551) monoclonal antibody recognizes the Y551-phosphorylated form of human Btk and the Y511 phosphorylated form of human Itk.

Characteristics: 1. Please refer to us for technical protocols.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

Comments:

Related Products: ABIN967389

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4° C.

Restrictions: For Research Use only

Images

Western blot analysis of Btk (pY551) in human Burkitt’s lymphoma. Lysates from control (panel A) and pervanadate-treated (panel B) Raji cells (ATCC CCL-86) were probed with Purified Mouse anti-Btk (pY551)/Itk (pY511) monoclonal antibody at concentrations of 0. 5, 0.25, and 0.125 µg/ml. Btk (pY551) is identified as a band of about 77 kDa in the treated cells.

Publications

Product: Mahajan, Fargnoli, Burkhardt et al.: "Src family protein tyrosine kinases induce autoactivation of Bruton's tyrosine kinase." in: Molecular and cellular biology, Vol. 15, Issue 10, pp. 5304-11, 1995 (PubMed).

Rawlings, Scharenberg, Park et al.: "Activation of BTK by a phosphorylation mechanism initiated by SRC family kinases." in: Science (New York, N.Y.), Vol. 271, Issue 5250, pp. 822-5, 1996 (PubMed).

Wilcox, Berg: "Itk phosphorylation sites are required for functional activity in primary T cells." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37112-21, 2003 (PubMed).