B-Cell Linker (BLNK) (pTyr84) antibody

Details for Product No. ABIN967596
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Antigen
Synonyms BLNK, blnk, MGC147045, BASH, Bca, Ly-57, Ly57, Lyw-57, SLP-65, AGM4, BLNK-S, LY57, SLP65, bca
Epitope
pTyr84
(21), (20), (12), (10), (8), (7), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(126), (76), (52), (14), (14), (14), (1)
Host
Mouse
(113), (10), (9)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1)
Application
Western Blotting (WB)
(90), (47), (40), (34), (18), (8), (6), (5), (2), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN967596
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Immunogen Phosphorylated Human BLNK Peptide
Clone J117-1278
Isotype IgG2b, kappa
Cross-Reactivity Mouse (Murine)
Characteristics 1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name BLNK
Background B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The J117-1278 monoclonal antibody recognizes the phosphorylated Y84 of BLNK.
Molecular Weight 68 kDa
Research Area Signaling, Adaptive Immunity
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-B-Cell Linker (BLNK) (pTyr84) antibody Western blot analysis of BLNK (pY84) in human Burkitt’s lymphoma. Lysates from control (lanes 1-3) and hydrogen peroxide-activated (lanes 4-6) Ramos cells were probed with purified mouse anti-BLNK (pY84) monoclonal antibody) at concentrations of 0.125 (lanes 1 and 4), 0.0625 (lanes 2 and 5), and 0.032 µg/ml (lanes 3 and 6). BLNK (pY84) is identified as a band of about 68 kDa in the treated cells.
Product cited in: Chiu, Dalton, Ishiai et al.: "BLNK: molecular scaffolding through 'cis'-mediated organization of signaling proteins." in: The EMBO journal, Vol. 21, Issue 23, pp. 6461-72, 2002 (PubMed).

Janssen, Zhang: "Adaptor proteins in lymphocyte activation." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, 2003 (PubMed).

Wu, Koretzky: "The SLP-76 family of adapter proteins." in: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, 2004 (PubMed).

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