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B-Cell Linker (BLNK) (pTyr84) antibody

Antigen

B-Cell Linker (BLNK)
Synonyms Bca, BASH, Ly57, Ly-57, Lyw-57, SLP-65, LY57, SLP65, BLNK-S, MGC111051, BLNK, MGC112831, MGC127870, blnk, MGC147045
Binding Site
Alternatives

pTyr84
Clonality Monoclonal (J117-1278)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB)
3 references available
Catalog no. ABIN967596
Quantity 0.1 mg  (0.5 mg/ml)
Price Product not available in this region.
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anti-B-Cell Linker (BLNK) (pTyr84) antibody

Additional Information
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Alternative name BLNK
Immunogen Phosphorylated Human BLNK Peptide
Cross-Reactivity Mouse (Murine)
Format Liquid
Isotype IgG2b, kappa
Clone J117-1278
Description B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The J117-1278 monoclonal antibody recognizes the phosphorylated Y84 of BLNK.
Characteristics 1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Molecular Weight 68 kDa
Comments

Related Products: ABIN967389

Application Details
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Concentration 0.5 mg/ml
Purity Purified
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Buffer Aqueous buffered solution.
Preservative 0.09% Sodium azide.
Storage Store undiluted at 4°C.
Research Area Signaling, Adaptive Immunity
Restrictions For Research Use only

Publications
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Product Chiu, Dalton, Ishiai et al.: "BLNK: molecular scaffolding through 'cis'-mediated organization of signaling proteins." in: The EMBO journal, Vol. 21, Issue 23, pp. 6461-72, 2002 (PubMed).

Janssen, Zhang: "Adaptor proteins in lymphocyte activation." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, 2003 (PubMed).

Wu, Koretzky: "The SLP-76 family of adapter proteins." in: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, 2004 (PubMed).

Alternatives
Application Details Publications Images back to top

Alternatives for antigen "B-Cell Linker (BLNK)", type "Antibodies"
Hosts Rabbit (84), Goat (7), Mouse (2)
Reactivities Human (83), Mouse (Murine) (75), Rat (Rattus) (64), Cow (Bovine) (21), Dog (Canine) (19), Horse (Equine) (18), Pig (Porcine) (18), Cat (Feline) (1), Chicken (1)
Applications Immunofluorescence (IF) (53), Western Blotting (WB) (42), ELISA (28), Immunohistochemistry (IHC) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (9), Immunoprecipitation (IP) (4), Immunoelectron Microscopy (IEM) (3), Flow Cytometry (FACS) (2)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Biotin (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), FITC (3), Gold (3), HRP (3), PE (3), PE,Cy5.5 (2), PE-Cy3 (2), PE-Cy5 (2), PE-Cy5.5 (2), PE-Cy7 (2), Un-conjugated (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pTyr84 (19), pTyr491 (18), pTyr96 (6), AA 171-356 (5), C-Term (4), Internal Region (3), Center (1), N-Term (1), pThr56 (1)