Retinoblastoma 1 (RB1) (pSer807), (pSer811) antibody

Details for Product No. ABIN967607
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Synonyms RB1, OSRC, RB, p105-Rb, pRb, pp110, Rb, Rb-1, zgc:154147, P105-RB, PP110, RB11
pSer807, pSer811
(35), (35), (31), (23), (19), (19), (19), (17), (17), (15), (13), (7), (6), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(368), (229), (217), (109), (85), (65), (48), (7), (1), (1), (1)
(329), (52), (2)
Clonality (Clone)
Monoclonal ()
(10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10)
Western Blotting (WB)
(239), (110), (100), (92), (76), (49), (37), (32), (18), (11), (8), (8), (3), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN967607
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Immunogen Phosphorylated Human Rb Peptide
Clone J112-906
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Rb
Background The retinoblastoma gene product (Rb) is well known as a tumor suppressor and is either absent or mutated in many human tumors. Retrovirus-mediated gene transfer of the wild-type Rb gene into several Rb mutant neoplastic cell lines suppresses their tumorgenicity. Rb is a 110-kDa nuclear phosphoprotein that undergoes differential phosphorylation during the cell cycle. During G1 phase, Rb is predominantly in a hypophosphorylated state. It becomes increasingly phosphorylated throughout the cell cycle until late mitosis, when substantial dephosphorylation occurs. Hypophosphorylated Rb interacts with a number of cellular proteins including the E2F transcription factor, several cyclins, RBP-1, RBP-2, c-Abl, c-myc, N-myc, and p46. Phosphorylation of Rb at various sites, by Cyclin-dependent protein kinases, inhibits the binding of Rb to these proteins. Rb is thought to mediate its effects, in part, via the repression of genes required for proliferation. For example, Rb is specifically recruited to promoters containing E2F sites and actively represses E2F mediated transcription. Rb also stimulates the activity of other transcription factors, although the mechanisms are less clearly defined. Thus, Rb appears to regulate transcription in its aim to control cell growth.
The J112-906 monoclonal antibody recognizes Rb phosphorylated at serines 807 and 811 (pS807/pS811), which regulate c-Abl binding and cell cycle progression. The orthologous phosphorylation sites in mouse and rat Rb are pS800/pS804 and pS778/pS782, respectively.
Molecular Weight 110 kDa
Research Area Cancer, Transcription Factors

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Retinoblastoma 1 (RB1) (pSer807), (pSer811) antibody Western blot analysis of Rb (pS807/pS811) in human embryonic skin cells. Lysates from serum-starvedl (first panel) and fetal bovine serum-stimulated (second panel) WS1 cell line were probed with purified mouse anti-Rb (pS807/pS811) monoclonal antibody at concentrations of 0.0313 µg/mL (lanes 1 and 4), 0.0156 µg/mL (lanes 2 and 5), and 0.008 µg/mL (lanes 3 and 6). Rb (pS807/pS811) is identified as a band of 110 kDa in the stimulated cells.
Product cited in: Cobrinik: "Pocket proteins and cell cycle control." in: Oncogene, Vol. 24, Issue 17, pp. 2796-809, 2005 (PubMed).

Ren, Rollins: "Cyclin C/cdk3 promotes Rb-dependent G0 exit." in: Cell, Vol. 117, Issue 2, pp. 239-51, 2004 (PubMed).

Knudsen, Wang: "Differential regulation of retinoblastoma protein function by specific Cdk phosphorylation sites." in: The Journal of biological chemistry, Vol. 271, Issue 14, pp. 8313-20, 1996 (PubMed).

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