ROR GAM T antibody

Details for Product No. ABIN967684
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Antigen
Reactivity
Human
Host
Mouse
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
Pubmed 6 references available
Quantity 0.1 mg
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Catalog No. ABIN967684
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Immunogen Human RORgammat Recombinant Protein
Clone O28-835
Isotype IgG2b, kappa
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Background The O28-835 monoclonal antibody recognizes ROR-gamma (RORgamma) and its isoform, RORgammat. RORgamma (~58 kDa calculated molecular weight) is a DNA-binding transcription factor that belongs to the ROR/RZR orphan nuclear receptor family. It is expressed in different tissues including skeletal muscle, kidney, liver and adipose tissues. RORgammat (~56 kDa calculated molecular weight) is a RORgamma isoform that is expressed exclusively by lymphoid cells. It is expressed by various lymphoid cell types including CD4+CD8+ thymocytes, peripheral CD4+ Th17 and CD8+ Tc17 cells and lymphoid tissue inducer (Lti) cells. RORgammat plays essential roles in thymopoiesis, T cell homeostasis, differentiation of effector T lymphocytes and the development of secondary lymphoid tissues including lymph nodes and Peyer’s patches.
Synonyms: Rorc, Rorg, RORgamma, Rorgamma, RORgt, RORgammat, RORgammat, TOR, Thor, Nr1f3, ROR
Molecular Weight 56–58 kDa
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-ROR GAM T antibody Western blot analysis of RORgammat expression by mouse thymocytes. Cell lysate was prepared from untreated C57BL/6 mouse thymus (lanes 1-3). The lysate was electrophoresed (SDS-PAGE, approximately 12.5 myg of total cellular protein per lane), transferred to a PVDF membrane and then probed with Purified Mouse Anti-RORgammat antibody (Clone O28-835, ABIN967684) at concentrations of 1.25 (lane 1), 0.6 (lane 2), and 0.3 µg/ml (lane 3). Proteins were detected using HRP Goat Anti-Mouse Ig (ABIN967389) and a chemiluminescent detection system. RORgt is identified as a band of ~56 kDa in the thymus cell lysate.
Product cited in: Medvedev, Yan, Hirose et al.: "Cloning of a cDNA encoding the murine orphan receptor RZR/ROR gamma and characterization of its response element." in: Gene, Vol. 181, Issue 1-2, pp. 199-206, 1997 (PubMed).

He, Deftos, Ojala et al.: "RORgamma t, a novel isoform of an orphan receptor, negatively regulates Fas ligand expression and IL-2 production in T cells." in: Immunity, Vol. 9, Issue 6, pp. 797-806, 1999 (PubMed).

Eberl, Littman: "The role of the nuclear hormone receptor RORgammat in the development of lymph nodes and Peyer's patches." in: Immunological reviews, Vol. 195, Issue 9, pp. 81-90, 2003 (PubMed).

Eberl, Marmon, Sunshine et al.: "An essential function for the nuclear receptor RORgamma(t) in the generation of fetal lymphoid tissue inducer cells." in: Nature immunology, Vol. 5, Issue 1, pp. 64-73, 2003 (PubMed).

Ivanov, McKenzie, Zhou et al.: "The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells." in: Cell, Vol. 126, Issue 6, pp. 1121-33, 2006 (PubMed).

Hamada, Garcia-Hernandez, Reome et al.: "Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 6, pp. 3469-81, 2009 (PubMed).

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