Insulin Receptor antibody (AA 1006-1144)
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- Target See all Insulin Receptor (INSR) Antibodies
- Insulin Receptor (INSR)
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Binding Specificity
- AA 1006-1144
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Reactivity
- Mouse, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Insulin Receptor antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
- Cross-Reactivity
- Mouse (Murine), Rat (Rattus)
- Characteristics
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Human Insulin pro-receptor beta subunit 1006-1144
- Clone
- 46-CD220
- Isotype
- IgG1
- Top Product
- Discover our top product INSR Primary Antibody
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- Comment
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Related Products: ABIN967389
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 250 μg/mL
- Buffer
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store undiluted at -20° C.
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pp60Src mediates insulin-stimulated sequestration of the beta(2)-adrenergic receptor: insulin stimulates pp60Src phosphorylation and activation." in: Molecular biology of the cell, Vol. 13, Issue 11, pp. 3943-54, (2002) (PubMed).
: "Caveolin 1-mediated regulation of receptor tyrosine kinase-associated phosphatidylinositol 3-kinase activity by ceramide." in: Molecular and cellular biology, Vol. 20, Issue 5, pp. 1507-14, (2000) (PubMed).
: "Insulin/IGF-1 hybrid receptors: implications for the dominant-negative phenotype in syndromes of insulin resistance." in: Journal of cellular biochemistry, Vol. 48, Issue 1, pp. 43-50, (1992) (PubMed).
: "Insulin receptors are bivalent as demonstrated by photoaffinity labeling." in: The Journal of biological chemistry, Vol. 267, Issue 19, pp. 13131-4, (1992) (PubMed).
: "The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling." in: Cell, Vol. 40, Issue 4, pp. 747-58, (1985) (PubMed).
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pp60Src mediates insulin-stimulated sequestration of the beta(2)-adrenergic receptor: insulin stimulates pp60Src phosphorylation and activation." in: Molecular biology of the cell, Vol. 13, Issue 11, pp. 3943-54, (2002) (PubMed).
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- Target
- Insulin Receptor (INSR)
- Alternative Name
- CD220 (INSR Products)
- Synonyms
- CD220 antibody, HHF5 antibody, 4932439J01Rik antibody, D630014A15Rik antibody, IR antibody, IR-A antibody, IR-B antibody, 18402 antibody, CG18402 antibody, DIHR antibody, DILR antibody, DIR antibody, DIRH antibody, DIRbeta antibody, DInR antibody, DInr antibody, Dir-a antibody, Dir-b antibody, Dmel\\CG18402 antibody, INR antibody, INS antibody, Inr antibody, Inr-alpha antibody, Inr-beta antibody, InsR antibody, dINR antibody, dIR antibody, dIRH antibody, dInR antibody, dInr antibody, dInsR antibody, dinr antibody, dir antibody, er10 antibody, inr antibody, insulin/insulin-like growth factor receptor antibody, l(3)05545 antibody, l(3)93Dj antibody, l(3)er10 antibody, lnR antibody, ir-A antibody, CTK-1 antibody, ir antibody, INSR antibody, NV14476 antibody, cd220 antibody, hhf5 antibody, insulin receptor antibody, Insulin-like receptor antibody, insulin receptor L homeolog antibody, INSR antibody, Insr antibody, InR antibody, LOC100122567 antibody, LOC100451802 antibody, insr.L antibody
- Background
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Insulin Receptor (IR) is a transmembrane receptor tyrosine kinase which, upon insulin binding, initiates a cascade of events, including autophosphorylation, phosphorylation of cellular protein substrates, glucose transport, and glycogen synthesis. IR is synthesized as a large glycosylated precursor that is cleaved upon maturation into a 130 kDa alpha-subunit with kinase activity and a 95 kDa beta-subunit. The active Insulin Receptor is a heterotetramer of homologus alpha and beta subunits joined by disulfide bonds. Among the major cytosolic substrates of the Insulin Receptor are IRS-1 and -2, beta-Adrenergic receptor, and pp15 (adipocyte lipid-binding protein, ALBP). Autophosphorylation of the IR recruits IRS-1 and -2 to the phosphotyrosines. Subsequently, the phosphorylated IRS-1 and -2 act as docking sites for other signaling proteins like PI3-Kinase, Shc, PTP1D, Nck, etc. In addition, the phosphatase LAR is tightly associated with the IR and LAR becomes activated after insulin stimulation dephosphorylating the IR and its substrates. Therefore, LAR provides a turn-off mechanism in insulin signaling. This antibody is routinely tested by western blot analysis.
Synonyms: Insulin Receptor beta - Molecular Weight
- 95 kDa
- Pathways
- NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity
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